ISSN:
1432-072X
Keywords:
Clostridium thermoaceticum
;
CO2 utilization
;
Hydrogenase
;
Autotrophism
;
Acetogenic bacteria
;
Acetyl-CoA synthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract It has been demonstrated that enzymes from Clostridium thermoaceticum catalyze the following reaction in which Fd is ferredoxin and CH3THF is methyltetrahydrofolate. $$\begin{gathered}{\text{CO}}_{\text{2}} {\text{ + H}}_{\text{2}} {\text{ + CH}}_{\text{3}} {\text{THF + }} {\text{CoASH}}\xrightarrow{{{\text{ATP, Fd}}}} \hfill \\{\text{CH}}_{\text{3}} {\text{COSCoA + THF + H}}_{\text{2}} {\text{O}}{\text{.}} \hfill \\\end{gathered}$$ The system involves hydrogenase, CO dehydrogenase, a methyltransferase, a corrinoid enzyme and other unknown components. Hydrogenase catalyzes the reduction of ferredoxin by H2; CO dehydrogenase then uses the reduced ferredoxin to reduce CO2 to a one-carbon intermediate that combined with CoASH and with a methyl group originating from CH3THF to form acetyl-CoA. It is proposed that these reactions are part of the mechanism which enables certain acetogenic autotrophic bacteria to grow on CO2 and H2.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425809
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