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  • 1
    ISSN: 1573-5001
    Keywords: Pulsed-field-gradient NMR ; Translational diffusion coefficient ; Self-association ; Myosin light chain ; CHAPS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary At the millimolar concentrations required for structural studies, NMR spectra of the calcium-binding protein myosin light chain 2 (MLC2) showed resonance line widths indicative of extensive self-association. Pulsed-field-gradient (PFG) NMR spectroscopy was used to examine whether MLC2 aggregation could be prevented by the zwitterionic bile salt derivative 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS). PFG NMR measurements indicated that CHAPS was capable of preventing MLC2 self-association, but only at concentrations well above the critical micelle concentration of ∼7.5 mM. CHAPS was most effective at a concentration of 22.5 mM, where the apparent molecular mass of MLC2 correponded to a protein monomer plus seven molecules of bound detergent. The resolution and sensitivity of 2D 15N-1H HSQC spectra of MLC2 were markedly improved by the addition of 25 mM CHAPS, consistent with a reduction in aggregation following addition of the detergent. The average amide nitrogen T2 value for MLC2 increased from ∼30 ms in the absence of CHAPS to ∼56 ms in the presence of 25 mM CHAPS. The results of this study lead us to propose that PFG NMR spectroscopy can be used as a facile alternative to conventional techniques such as analytical ultracentrifugation for examining the self-association of biological macromolecules.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of agricultural and environmental ethics 13 (2000), S. 205-217 
    ISSN: 1573-322X
    Keywords: Animal welfare ; Australia ; blowfly ; fly strike ; New Zealand ; sheep ; United Kingdom
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Philosophy
    Notes: Abstract Fly strike is a painful conditioncaused by live maggots eating at the flesh of sheep.Remedies for this disorder are traumatic, with sheepundergoing painful mulesing and tail dockingoperations to protect against flystrike. In an attemptto find control solutions and to understand thedisorder, Australasian researchers increase sheepsuffering by conducting experiments that artificiallyinduce fly strike. Some of these experiments have noapplication in prevention and control of fly strike.Many others could be modified or replaced with lesspainful techniques. Anecdotal evidence through communication withorganic farmers suggests that fly strike is largelypreventable if farmers keep sheep healthy and inspectthem regularly. Some organic farmers have largelyeliminated fly strike from their farm. Investigationson fly strike control using non-intrusive techniquesare also progressing in Australasia and the UnitedKingdom. Since it is possible to conduct useful research andto run profitable farms with little or no fly strike,much of the current management, research and policy onsheep farming is ethically questionable even to thoseholding the moderate view that animals can be used forhuman purposes providing suffering is kept to aminimum. Sheep farmers will need to take animalwelfare problems into consideration. Greatercommunication among researchers needs to be encouragedto prevent unnecessary duplication of experiments.International trade regulations will also need toallow trade barriers based on animal welfareconcerns.
    Type of Medium: Electronic Resource
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