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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 28 (1995), S. 267-279 
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; C2H2 zinc finger protein ; transcription factor ; organ-specific expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Work from animal systems indicates that C2H2 zinc finger proteins play an important role in development, and this is likely true for plant systems as well. To address this question we have used the sequence information from a previously isolated Arabidopsis thaliana C2H2 zinc finger protein gene to isolate additional cDNAs belonging to this gene family. While zinc finger genes isolated from other organisms encode multiple copies of this domain, the eight cDNAs isolated from Arabidopsis each contain only a single zinc finger. Outside of the finger region there is little sequence identity or similarity, although features characteristic of transcription factors are evident. While these genes are related, hybridization analysis indicates that each of them is a unique gene in the Arabidopsis genome. Analysis of mRNA demonstrates that the genes are expressed in different but overlapping sets of organs in the plant. These results are discussed in the light of recent analysis of zinc finger genes from other plant systems.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 32 (1996), S. 785-796 
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; β-glucuronidase ; C2H2 zinc finger protein ; histochemical analysis ; tissue-specific expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract C2H2 zinc finger protein genes encode nucleic acid-binding proteins involved in the regulation of gene activity. AtZFP1 (Arabidopsis thaliana zinc finger protein 1) is one member of a small family of C2H2 zinc finger-encoding sequences previously characterized from Arabidopsis. The genomic sequence corresponding to the AtZFP1 cDNA has been determined. Molecular analysis demonstrates that AtZFP1 is a unique, intronless gene which encodes a 1100 nucleotides mRNA highly expressed in roots and stems. A construct in which 2.5 kb of AtZFP1 upstream sequences is linked to the β-glucuronidase gene was introduced into Arabidopsis by Agrobacterium-mediated transformation of roots. Histochemical analysis of transgenic Arabidopsis carrying the AtZFP1 promotor:β-glucuronidase fusion shows good correlation with RNA blot hybridization analysis. This transgenic line will be a useful tool for analyzing the regulation of AtZFP1 to further our understanding of its function.
    Type of Medium: Electronic Resource
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