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  • 1
    Electronic Resource
    Electronic Resource
    1H resonance assignments, secondary structure and general topology of single-chain monellin in solution as determined by1H 2D-NMR (1992)
    Springer
    Journal of biomolecular NMR 2 (1992), S. 557-572 
    Details
    ISSN: 1573-5001
    Keywords: Monellin ; Sweet protein ; 1H assignments ; Engineered loop
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary We determined the resonance assignments, secondary structure and general topology of the 11-kDa sweet protein single-chain monellin (SCM), using two-dimensional proton nuclear magnetic resonance spectroscopy (2D-NMR). SCM is a genetically engineered protein whose design is based on the crystal structure of natural, two-chain monellin (Kim et al., 1989). Analysis of the NMR spectra shows that the secondary structure of SCM consists of a five-strand anti-parallel β-sheet and a 15-residue α-helix. Tertiary NOE constraints place the a-helix on the hydrophobic side of the β-sheet, and indicate that the sheet is partially wrapped around the helix. The general structural features determined for SCM are similar to those of native monellin (Ogata et al., 1987). Some differences between the SCM structure in solution and the crystal structure of monellin are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02192845
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  • 2
    Electronic Resource
    Electronic Resource
    Identification and neuroanatomical distribution of immunoreactivity for mammalian gonadotropin-releasing hormone (mGnRH) in the brain and neural hypophyseal lobe of the toad Bufo arenarum (1998)
    Springer
    Cell & tissue research 293 (1998), S. 419-425 
    Details
    ISSN: 1432-0878
    Keywords: Key words Gonadotropin-releasing hormone ; Brain ; Hypophyseal neural lobe (pars nervosa) ; Reproduction ; Bufo arenarum (Anura)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The presence and distribution of gonadotropin-releasing hormone (GnRH) in sexually mature specimens of Bufo arenarum was studied by reverse phase/high performance liquid chromatography (RP-HPLC) combined with radioimmunoassay and immunocytochemistry. The analysis of brain extracts with RP-HPLC followed by radioimmunoassay with PBL#45 antiserum showed the presence of only one peak with immunoreactivity for GnRH (ir-GnRH) having the chromatographic and immunological characteristics of mammalian GnRH (mGnRH). This peak was further analyzed with two mGnRH-specific antisera, EL-15 and m1076, yielding serial dilution displacement curves parallel to those obtained with the mGnRH synthetic standard. Immunocytochemical results with the monoclonal antibody LRH13 showed the presence of a terminal nerve-septo-preoptic system with neurons and fibers distributed from the olfactory bulb, septal area, and anterior preoptic area toward the hypothalamus and hypophyseal neural lobe. The main group of ir-GnRH fibers and neurons was identified in the anterior preoptic area. These neurons appear to be the origin of fibers that, after surrounding the preoptic recess, border the dorsal surface of the optic chiasma, extend through the infundibulum, traverse the external layer of the median eminence, and end in the pars nervosa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410051133
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