Publication Date:
1995-01-13
Description:
Peptides from endogenous proteins are presented by major histocompatibility complex class I molecules, but antigens (Ags) in the extracellular fluids are generally not. However, pathogens or particulate Ags that are internalized into phagosomes of macrophages (M phi s) stimulate CD8 T cells. The presentation of these Ags is resistant to chloroquine but is blocked by inhibitors of the proteasome, a mutation in the TAP1-TAP2 transporter, and brefeldin A. Moreover, phagocytosis of a ribosomal-inactivating protein inhibited M phi protein synthesis. These results demonstrate that M phi s transfer Ags from phagosomes into the cytosol and that endogenous and exogenous Ags use a final common pathway for class I presentation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kovacsovics-Bankowski, M -- Rock, K L -- AI20248/AI/NIAID NIH HHS/ -- AI31337/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1995 Jan 13;267(5195):243-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Lymphocyte Biology, Dana-Farber Cancer Institute, Boston, MA 02115.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7809629" target="_blank"〉PubMed〈/a〉
Keywords:
ATP-Binding Cassette Transporters/genetics/metabolism
;
Amino Acid Sequence
;
Animals
;
*Antigen Presentation
;
Antigens/*metabolism
;
Brefeldin A
;
Cell Line
;
Chloroquine/pharmacology
;
Cyclopentanes/pharmacology
;
Cysteine Endopeptidases/metabolism
;
Cytosol/*immunology/metabolism
;
Histocompatibility Antigens Class I/*immunology
;
Macrophages/*immunology
;
Mice
;
Molecular Sequence Data
;
Multienzyme Complexes/metabolism
;
Oligopeptides/immunology/metabolism
;
Ovalbumin/immunology/metabolism
;
Phagocytosis
;
Phagosomes/*immunology/metabolism
;
Plant Proteins/pharmacology
;
Proteasome Endopeptidase Complex
;
Ribosome Inactivating Proteins, Type 1
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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