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  • 1
    Electronic Resource
    Electronic Resource
    PCR detection of transcripts homologous to the self-incompatibility gene in anthers ofBrassica (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 466-472 
    Details
    ISSN: 1432-2242
    Keywords: Brassica oleracea ; Self-incompatibility ; S-locus ; Polymerase chain reaction ; Anther
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The polymerase chain reaction (PCR) is particularly well suited for the detection of rare sequences. Taking advantage of the recent isolation of sequences associated with stigma self-incompatibility inBrassica oleracea, we used PCR amplifications with primers synthesized to the S6 cDNA sequence, to demonstrate the presence of mRNA homologous to stigmaS-locus gene (SLG) in anthers during early microsporogenesis. In addition, otherS-locus-related (SLR) sequences were shown to be transcribed in sexual as well as in vegetative tissues (roots, leaves), suggesting that the SLG family might be involved not only in pollen-stigma recognition, but more generally in various forms of plant cell signalling processes. This information corroborates the recent discovery of a cDNA-deduced protein kinase from maize roots, whose extracellular receptor displays high homology withBrassica S-locus-specific glycoproteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00588600
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of carbon dioxide and relative humidity on self incompatibility in cauliflower, Brassica oleracea (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 628-633 
    Details
    ISSN: 1432-2242
    Keywords: Carbon dioxide ; Relative humidity ; Brassica oleracea ; Pollen-pistil interactions ; Self compatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The events of the progamic phase of fertilization have been monitored by in vitro experiments in self compatible (SC), partial self-incompatible (PSI) and self incompatible (SI) lines. The duration of the progamic phase is about 30 h. Treatment with low concentrations of CO2 (3 to 5%) at high relative humidity (rH, 100%) had the following effects: pollen quality, which declines normally during flower ageing, was prematurely reduced; pollen adhesion and germination, both low in SI matings, were increased; the stigma callose response in SI matings was reduced to the low level of SC matings; and the number of pollen tubes in the style after SI matings significantly increased. CO2 concentrations of 4 to 6% applied for 8, 16 or 24 h at 100% rH proved to be the most effective treatment for blocking the SI response in cauliflower.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252288
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  • 3
    Electronic Resource
    Electronic Resource
    Use of immunochemical and SSCP analyses to test homozygosity at theS locus ofBrassica oleracea genotypes (1995)
    Springer
    Molecular breeding 1 (1995), S. 237-244 
    Details
    ISSN: 1572-9788
    Keywords: Brassica oleracea ; immunochemical analysis ; self-incompatibility ; S locus glycoprotein ; SSCP ; stigma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The self-incompatibility reaction of cruciferous plants prevents self-fertilization and has been shown to be controlled by at least two genes situated at a single multiallelic locus, theS locus. One of these two genes, theS locus glycoprotein (SLG) gene, encodes an abundant glycoprotein secreted to the cell wall of stigma papillae. Identification of thoseS alleles present at theS locus is of prime interest when studying the self-incompatibility response and can be achieved by identifying the SLG of the stigma. Here, we show that using anti-SLG antibodies in an immunochemical analysis, combined with a SSCP (single-strand conformation polymorphism) approach to characterize the corresponding stigma-specific, SLG mRNA, allowed the identification of plants heterogeneous at theS locus among populations of plants that were thought to be homozygous for known SLG alleles. This analysis stresses the importance of testing the homozygosity at theS locus of lines considered inbred for a knownS allele as mix-up of seeds may occur during the breeding programme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02277424
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