ISSN:
1432-2242
Keywords:
Brassica
;
Mitochondrial DNA
;
Chloroplast DNA
;
Protoclone
;
Protoplast fusion
;
Anther culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Over 400 Brassica napus plants regenerated from individual protoplasts, from protoplast fusions and from anther culture were analysed for chloroplast and mitochondrial genome rearrangements by restriction fragment length polymorphisms. None were detected, attesting to the fidelity of the tissue culture procedures employed. In the majority of protoplast fusion products, the cytoplasmic organelles had completely sorted out at the callus stage but three regenerated plants possessed mixed parental populations of mitochondrial genomes and one regenerant contained mixed chloroplast genomes. In all four examples, the cytoplasmic genome sorted out in planta in favor of one parental type which was faithfully maternally transmitted to progeny.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00258048
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