ISSN:
1573-5028
Keywords:
Glycine max
;
Bowman-Birk protease inhibitors
;
in vitro translation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Poly (A)+ RNAs from immature soybean seeds were size fractionated in denaturing sucrose gradients to identify mRNA that directs the cell-free synthesis of the Bowman-Birk protease inhibitor and the related inhibitors PI I–IV. Polypeptides synthesized in vitro were labeled with (35S)-cysteine and (3H)-serine and detected by immunoprecipitation with anti Bowman-Birk and anti PI I–IV sera. Immunoprecipitates of the translation products comigrated on SDS-polyacrylamide gels with the dimeric or trimeric aggregates of the authentic inhibitor proteins, which self-associate under certain conditions. Further evidence that these immunoprecipitates contained authentic polypeptides corresponding to the Bowman-Birk or PI IV inhibitor was shown by sequential amino acid analyses of peptides generated by cleavage with cyanogen bromide.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00021034
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