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  • Bone resorption  (6)
  • Springer  (6)
  • American Geophysical Union (AGU)
Collection
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  • Springer  (6)
  • American Geophysical Union (AGU)
Years
  • 1
    Electronic Resource
    Electronic Resource
    Cyclo-oxygenase products of arachidonic acid metabolism in rat osteoblasts in culture (1983)
    Springer
    Calcified tissue international 35 (1983), S. 294-297 
    Details
    ISSN: 1432-0827
    Keywords: Prostaglandins ; Osteoblasts ; Bone resorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The metabolism of arachidonic acid to its cyclo-oxygenase products was studied in monolayer cultures of osteoblast-rich rat calvarial cells and of clonal cell lines from a rat osteogenic sarcoma, enriched in the osteoblast phenotype. Prostanoids were measured by radioimmunoassay after extraction of media and fractionation by high pressure liquid chromatography. In both normal and malignant osteoblasts the major cyclo-oxygenase product was 6-oxo-prostaglandin F1α, the hydration product of prostacyclin, with lesser amounts of prostaglandin E2 and prostaglandin F2α. No significant thromboxane B2 was detected. Prostaglandins are thought to have a local role in the regulation of bone resorption. These results point to the possible importance of prostacyclin either in bone resorption or in some other local function, e.g., regulation of bone blood flow.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405049
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  • 2
    Electronic Resource
    Electronic Resource
    Promethazine inhibits osteoclastic bone resorption In vitro (1994)
    Springer
    Calcified tissue international 55 (1994), S. 68-70 
    Details
    ISSN: 1432-0827
    Keywords: Promethazine ; Osteoclast ; Bone resorption ; H1 blockers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Several studies have shown that promethazine can reduce age-related osteopenia in mice. Furthermore, prolonged treatment with promethazine (50 mg/day) increases bone mineral content in the lumbar spine in post-menopausal women with osteopenia. However, the mechanism of action of promethazine has not been elucidated. The present study shows that promethazine HCl (0.01 – 10 μM) dose-dependently inhibits bone resorption by isolated rat osteoclasts in the bone slice assay with an IC50 of ∼1 μM. Since these concentrations are likely to be achieved in vivo, it is suggested that the beneficial effect of promethazine on osteopenia is at least partly due to a direct inhibitory effect on osteoclast activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310171
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  • 3
    Electronic Resource
    Electronic Resource
    Wortmannin, a potent inhibitor of phosphatidylinositol 3-kinase, inhibits osteoclastic bone resorption in vitro (1995)
    Springer
    Calcified tissue international 56 (1995), S. 336-338 
    Details
    ISSN: 1432-0827
    Keywords: Wortmannin ; Osteoclasts ; Phosphatidylinositol 3-kinase ; Bone resorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Phosphatidylinositol 3-kinase (Pl3-k) is involved in cellular signaling via the phosphoinositol pathway leading to mitogenesis in response to growth factors in proliferating cells, as well as cytoskeletal changes and secretory responses in terminally differentiated cells. The fungal metabolite, wortmannin, is a potent and selective inhibitor of Pl3-k at nanomolar concentrations. We show that wortmannin dose-dependently (0.001 – 1 μM) inhibits bone resorption by isolated rat osteoclasts in the bone slice pit assay with an IC50 of-5 nM. Wortmannin was not cytotoxic since osteoclast morphology and survival on bone slices was unaffected by concentrations up to 1 μM. Since primary osteoclasts are terminally differentiated cells and osteoclast cytoplasmic spreading and morphology was unaffected by wortmannin, we suggest that Pl3-k signaling is involved in vesicle exocytosis and ruffled border membrane formation that are required for osteoclastic bone resorption to take place.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318056
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  • 4
    Electronic Resource
    Electronic Resource
    Hydrochlorothiazide inhibits osteoclastic bone resorption In vitro (1994)
    Springer
    Calcified tissue international 55 (1994), S. 266-268 
    Details
    ISSN: 1432-0827
    Keywords: Hydrochlorothiazide ; Osteoclast ; Bone resorption ; Carbonic anhydrase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract Long-term thiazide diuretic use is associated with higher bone mineral density and reduced hip fracture rates, which are attributed to increased serum calcium levels and decreased parathyroid activity that lead to decreased bone resorption. The present study shows that 1–100 μM hydro-chlorothiazide (HCTZ) dose dependently inhibits bone resorption by isolated rat osteoclasts in the bone slice assay with an IC50 of ∼20 μM. At these concentrations, HCTZ did not affect osteoclast survival on bone slices and had no effect on the proliferation of UMR-106 rat osteoblasts, indicating that the compound is not cytotoxic. However, such concentrations of HCTZ are unlikely to be achieved in man where therapeutic doses are usually 12.5–100 mg/day. That the in vitro effect of HCTZ on bone resorption may be due to inhibition of osteoclast carbonic anhydrase is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310404
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  • 5
    Electronic Resource
    Electronic Resource
    Failure of cells of the mononuclear phagocyte series to resorb bone (1984)
    Springer
    Calcified tissue international 36 (1984), S. 556-558 
    Details
    ISSN: 1432-0827
    Keywords: Osteoclast ; Bone resorption ; Mononuclear phagocytes ; Monocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Monocytes, peritoneal macrophages, inflammatory polykaryons, and myeloid cell lines were incubated on slices of human cortical bone and assessed for their capacity to resorb bone by scanning electron microscopy. None of these cell types, mononuclear or multinucleate, induced any detectable change in the bone surface, even after prolonged incubation, and even in the presence of macrophage activators. These findings emphasise the inadequacies of mononuclear phagocytes as surrogate osteoclasts, and expose a discrepancy between45Ca release and bone resorption.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405365
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  • 6
    Electronic Resource
    Electronic Resource
    Mammalian collagenase predisposes bone surfaces to osteoclastic resorption (1985)
    Springer
    Cell & tissue research 241 (1985), S. 671-675 
    Details
    ISSN: 1432-0878
    Keywords: Collagenase ; Osteoclast ; Bone resorption ; Osteoblast ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cell-free endocranial surface of young adult rat parietal bones was used as a substrate for bone cell-derived mammalian collagenase. Incubation of parietal bones in a concentration of enzyme comparable to that secreted by osteoblastic cells in vitro caused destruction of surface osteoid, and resulted in exposure of mineral onto the bone surface. Bones so pre-treated were considerably more susceptible to osteoclastic resorption than bones preincubated in the absence of collagenase. These results are consistent with the view that the osteoid layer which covers bone surfaces acts as a barrier to osteoclastic contact with underlying, resorption — stimulating bone mineral; and that cells of the osteoblastic lineage induce osteoclastic resorption through collagenase secretion which, by digestion of the surface osteoid, exposes bone mineral to osteoclastic contact.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214590
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