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  • Biochemistry and Biotechnology  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Expression of tumor-associated protein variants in chemically induced rat hepatomas and transformed rat liver cell lines determined by two-dimensional electrophoresis (1994)
    Weinheim : Wiley-Blackwell
    Electrophoresis 15 (1994), S. 372-381 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: High resolution two-dimensional electrophoresis (2-DE), using the rat liver as a model, was applied to study hepatocarcinogenesis induced by different chemical carcinogens. Several tumor-associated protein variants were detected by 2-DE in chemically induced rat hepatomas and transformed rat liver cell lines compared to normal rat liver tissue. Proliferation-related protein changes and/or protein alterations due to culture conditions were corrected for by comparison with 2-DE patterns of isolated cells from regenerating liver and short-term cultivated liver cells. Some of the tumor-associated variants were further characterized: (i) By peptide mapping with limited proteolysis we detected clear relationships between several variants. (ii) By studying posttranslational modifications phosphorylated and glycosylated variants could be determined. (iii) A tumor-associated protein variant was identified by amino acid analysis and amino acid sequencing.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150150156
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  • 2
    Electronic Resource
    Electronic Resource
    A new method to assign immunodetected spots in the complex two-dimensional electrophoresis pattern (1997)
    Weinheim : Wiley-Blackwell
    Electrophoresis 18 (1997), S. 799-801 
    Details
    ISSN: 0173-0835
    Keywords: Two-dimensional polyacrylamide gel electrophoresis ; Immunoblotting ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A new, easy method for the immunodetection of specific antigens in two-dimensional electrophoresis (2-DE) is described. Areas of 2-DE gels containing antigens of interest are electrophoretically transferred to polyvinylidene difluoride membranes, immunostained with specific antibodies using Fast Red or 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium as detection systems and counterstained with Coomassie Brilliant Blue. In contrast to conventional methods, it is possible to use this procedure to exactly assign immunoreacting proteins on a single blot to their corresponding and surrounding blue-stained protein spots.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150180524
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