ISSN:
0173-0835
Keywords:
Pulsed-field capillary electrophoresis
;
Hydroxyethyl cellulose
;
Polyethylene oxide
;
Restriction fragments
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Pulsed-field capillary electrophoresis (PFCE) in buffers containing ultradilute polymer solutions in used to separate long chain dsDNA in less than 4 min. Separations are shown to work with chain lengths below 10 kbp and greater than 1.5 Mbp. Several pulse protocols have been examined. If running time is to be minimized, a field inversion with higher peak amplitude in the forward direction than in the reverse, but with equal pulse durations, provides the best resolution. Other protocols can provide higher resolution, but only with longer resolution. Other protocols can provide higher resolution, but only with longer running times.
Additional Material:
12 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150170125
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