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  • 1
    Electronic Resource
    Electronic Resource
    Fluorescence resonance energy transfer visualization of DNA aggregates formed during electrophoretic separations in ultradilute hydroxyethylcellulose (1996)
    Weinheim : Wiley-Blackwell
    Electrophoresis 17 (1996), S. 1110-1114 
    Details
    ISSN: 0173-0835
    Keywords: Capillary electrophoresis ; Hydroxyethylcellulose ; Energy transfer ; DNA aggregate ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Fluoresence resonance energy transfer (FRET) microscopy is used to observe structures of DNA aggregates formed during electrophoresis in ultradilute hydroxyethylcellulose (HEC). FRET gives evidence of aggregates with a tightly packed core and random entanglement of DNA in the outer portions. HEC concentrations also appear to play a role in aggregate formation. DNA aggregates take longer to assemble in higher HEC concentration solutions and assume a less compact form than those formed at lower HEC concentrations.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150170621
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  • 2
    Electronic Resource
    Electronic Resource
    Electric birefringence imaging of DNA in agarose electrophoresis gels: Noise limits and prolonged electric field effects (1994)
    Weinheim : Wiley-Blackwell
    Electrophoresis 15 (1994), S. 137-142 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Electric birefringence imaging (EBI) maps stresses in agarose electrophoresis gels. Both DNA and gel alignment can be monitored using this technique. Previously, we have reported birefringence images associated with resolved small (〈 48 kbp) and partially resolved large 48 kb - (2 Mbp) DNA fragments oriented with relatively short electric field pulses. In this paper, differences between images acquired using short and long (DC) voltage pulses are presented. It is shown that stress signals distribute over long distances when exposed to prolonged electric fields. Resolution also affects band shapes. Refinements in instrumental design and evaluation of limiting noise sources of the technique are also described.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150150123
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrafast high resolution separation of large DNA fragments by pulsed-field capillary electrophoresis (1996)
    Weinheim : Wiley-Blackwell
    Electrophoresis 17 (1996), S. 152-160 
    Details
    ISSN: 0173-0835
    Keywords: Pulsed-field capillary electrophoresis ; Hydroxyethyl cellulose ; Polyethylene oxide ; Restriction fragments ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Pulsed-field capillary electrophoresis (PFCE) in buffers containing ultradilute polymer solutions in used to separate long chain dsDNA in less than 4 min. Separations are shown to work with chain lengths below 10 kbp and greater than 1.5 Mbp. Several pulse protocols have been examined. If running time is to be minimized, a field inversion with higher peak amplitude in the forward direction than in the reverse, but with equal pulse durations, provides the best resolution. Other protocols can provide higher resolution, but only with longer resolution. Other protocols can provide higher resolution, but only with longer running times.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150170125
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