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  • 1
    Electronic Resource
    Electronic Resource
    Rapid analytical extraction of volatile fermentation products (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 21 (1979), S. 1881-1883 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260211016
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  • 2
    Electronic Resource
    Electronic Resource
    Mobile self-contained off-gas analysis units for fermentation pilot plants (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 22 (1980), S. 1273-1276 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260220613
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  • 3
    Electronic Resource
    Electronic Resource
    A simple hobby Computer-based off-gas analysis system (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 23 (1981), S. 455-460 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260230222
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  • 4
    Electronic Resource
    Electronic Resource
    Investigation of bacterial growth on mixed substrates: Experimental evaluation of cybernetic models (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 28 (1986), S. 1044-1055 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cybernetic models, developed earlier by the authors, have been evaluated experimentally for the growth of Klebsiella oxytoca in batch cultures using mixed substrates from glucose, xylose, arabinose, lactose, and fructose. Based entirely on information procured from batch growth on single substrates, the models accurately predict without further parameter fitting, diauxic growth on mixed substrates, automatically predicting the order in which the substrates are consumed. Even triauxic growth on a mixture of glucose, xylose, and lactose is predicted by the model based on single substrate data. Growth on glucose-fructose mixtures appears to need a slightly modified strategy for cybernetic variables.
    Additional Material: 18 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260280715
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  • 5
    Electronic Resource
    Electronic Resource
    Cybernetic modeling of bacteriol cultures at low growth rates: Single-substrate systems (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 34 (1989), S. 252-261 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The cybernetic framework developed by Ramkrishna and co-workers has been expanded to include the effects of cellular maintenance energy requirements on biomass levels in slow-growing, carbon-substrate-limited cultures. A simple structured model, based on the existence of distinct key enzymes for growth and maintenance functions, is presented. Comparisons of the model with experimental data for the growth of Klebsiella oxytoca in constant fed-batch culture on glucose, fructose, arabinose, and xylose show good agreement. In addition, perturbed fed-batch culture experiments indicate that slow-growing cultures respond less rapidly to a removal of the growth limitation than do faster-growing ones. The possibility of a growth-rate dependent “critical resource” is discussed.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260340214
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  • 6
    Electronic Resource
    Electronic Resource
    A sensitive CCD image system for detection of chemiluminescent reactions (1989)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 3 (1989), S. 53-57 
    Details
    ISSN: 0884-3996
    Keywords: Chemiluminescence ; peroxidase ; CCD camera ; image intensifier ; image processing ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A new ultra-sensitive light detection system is described for the detection of chemiluminescence reactions. The system consists of a CCD camera equipped with a two-stage image intensifier coupled to an image analysis system with real-time filtering capabilities. The sensitivity was determined with the enhanced chemiluminescence system using horseradish peroxidase as label. Additional possibilities for further increase in sensitivity and the potential use in immuoassays and other applications are discussed.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170030204
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  • 7
    Electronic Resource
    Electronic Resource
    Characteristics and detection principles of a new enzyme label producing a long-term chemiluminescent signal (1989)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 4 (1989), S. 129-135 
    Details
    ISSN: 0884-3996
    Keywords: Chemiluminescence ; enzyme ; xanthine oxidase ; long-term signal ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A new enzyme label system is described which is superior to all existing chemiluminescence labels used in immunoassays. The system consists of the enzyme xanthine oxidase with hypoxanthine as substrate. The signal reagent contains perborate, an Fe-EDTA complex and luminol. The enzyme preparation and the signal reagent are very stable upon storage. The main features of the system are a long duration of the chemiluminescent signal (half-life time of 30 hours) and a very low limit of detection (about 3 amol). Possibilities and implications for the use of various measuring system are discussed.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170040119
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  • 8
    Electronic Resource
    Electronic Resource
    Production of 2,3-butanediol from D-xylose by Klebsiella oxytoca ATCC 8724 (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 362-369 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: It is known that 2,3-butanediol is a potentially valuable chemical feedstock that can be produced from the sugars present in hemicellulose and celluose hydrolysates. Klebsiella oxytoca is able to ferment most pentoses, hexoses, and disaccharides. Butanediol appears to be a primary metabolite, excreted as a product of energy methabolism. The theoretical maximum yield of butanediol from monosaccharides is 0.50 g/g. This article describes the effects of pH, xylose concentration, and the oxygen transfer rate on the bioconversion of D-xylose to 2,3-butanediol. Product inhibition by butanediol is also examined. The most important variable affecting the kinetics of this system appears to be the oxygen transfer rate. A higher oxygen supply favors the formation of cell mass at the expense of butanediol. Decreasing the oxygen supply rate increases the butanediol yield, but decreases the overall conversion rate due to a lower cell concentration.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260260411
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  • 9
    Electronic Resource
    Electronic Resource
    Application of bioenergetics to modelling the microbial conversion of D-xylose to 2,3-butanediol (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 573-582 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: During the oxygen limiting growth of Klebsiella oxytoca, the xylose metabolism may be considered as consisting of three components: conversion to 2,3-butanediol by “fermentation,” oxidation to carbon dioxide by respiration, and assimilation to cell mass. The amount of energy required for the assimilation of cell mass is assumed to determine the extent to which the two energy producing reactions occur. The activity of each energy producing pathway is also determined by the availability of oxygen and by the energy yield of each pathway. These relationships can be quantified by equating the ATP required for growth and maintenance to the ATP produced by the energy producing reactions. The resulting equation for butanediol production appears similar to the Luedeking and Piret model where the parameters α and β are related to the maximum cell yield from ATP and the maintenance energy requirement. These parameters were estimated from 14 batch fermentations, and the resulting simulation was used to describe the effects of the oxygen transfer rate and the initial xylose concentration on the yields and rates of the 2,3-butanediol fermentation.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260260603
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  • 10
    Electronic Resource
    Electronic Resource
    Performance of the duckweed species Lemna gibba on municipal wastewater for effluent renovation and protein production (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 29 (1987), S. 258-268 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An outdoor experiment was conducted in miniponds to evaluate the performance of Lemna gibba, a duckweed species, as a domestic wastewater stripper. Duckweed is one of the floating plants with a high capability of ammonia uptake and assimilation rate into valuable protein. The results indicate that under adequate operational conditions, depending mainly on the organic loading, the effluent quality meets irrigation reuse criteria and protein yield of the duckweed may reach 12 ton/ha per year, far above other conventional field crops.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260290217
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