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  • Biochemistry and Biotechnology  (1)
  • Chemical Engineering  (1)
  • MATHEMATICS AND INFORMATION SCIENCES  (1)
  • 1
    ISSN: 0006-3592
    Keywords: CHO cells ; serum-free medium ; adaptation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Using an adaptive strategy, Chinese hamster ovary (CHO) cell lines were developed that are capable of robust growth in serum-free suspension culture. These preadapted derivatives of the commonly used strain of CHO cells (CHO DUKX), termed PA-DUKX, were used for the introduction and stable expression of several heterologous human genes. A significant advantage of recombinant PA-DUKX cells was their ability to readily resume growth in serum-free suspension culture after transfection and amplification of heterologous genes. Expression of recombinant human proteins in PA-DUKX cells was quantitatively similar to that of lineages generated using conventional CHO DUKX cells. In addition, recombinant human proteins expressed by transfected PA-DUKX lineages were shown to be biochemically and structurally similar to those expressed in CHO DUKX cells, PA-DUKX host cell technology provides an opportunity for reducing the time and resources required to develop large-scale, suspension culture-based manufacturing processes employing serum-free medium. © 1996 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Brookfield, Conn. : Wiley-Blackwell
    Polymer Composites 10 (1989), S. 313-321 
    ISSN: 0272-8397
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper deals with the mechanical performances of a new class of injection molded long fiber composites based on PP and PBT matrices. Effects of material parameters such as fiber concentration, breakage, orientation, and matrix composition are analyzed. The critical fiber length, lεc, of the PP long fiber composite, evaluated from the pull-out length of the tensile fracture surface, was found to be much higher than those previously reported. Tensile strength calculated from the measured llεc and fiber length distribution in the molded samples was found to be in agreement with the measured values. From this work it is concluded that higher mechanical performances of the long fiber reinforced thermoplastics will be attained by the injection molding process to further reduce fiber breakage.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2019-06-27
    Description: Computer program can be used to analyze any disk or shell of revolution of arbitrary cross section under inertial loads caused by rotation about shell axis and under various static loads, including thermal gradients. Geometric shapes incorporated in program are ellipsoidal, spherical, ogival, toroidal, conical, circular plate, cylindrical, and parabolic.
    Keywords: MATHEMATICS AND INFORMATION SCIENCES
    Type: MFS-21970
    Format: application/pdf
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