ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Thin layer chromatography
;
Sample pre-treatment
;
Bile salts
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The analysis of bile acids in biological matrices, e.g. gall bladder bile and serum, depends on accurate and reproducible sample pre-treatment techniques. The present study compares published and commonly used protocols with a special focus on the analysis of serum bile acids. Since adsorption of bile acids to serum proteins has a dramatic impact on the recovery rate and the reproducibility of the method used, several deproteination procedures were compared. The combination of given protocols made it possible to develop a very sensitive and efficient sample pre-treatment which is well suited for all chromatographic methods for bile acid analysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02467599
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