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  • 1
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 25 (1991), S. viii 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Cytoplasmic male sterility ; mtDNA ; Restriction fragment patterns ; Filter hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial (mt) DNA from eight cytoplasmic male-sterile (cms) lines of sugar beet from different breeding stations was investigated by restriction fragment analysis and Southern hybridization. All cms lines showed similar but not identical restriction and hybridization signal patterns, readily distinguishable from those of fertile (N) cytoplasm. Digestion of the mtDNA with BamHI, EcoRI, SalI, and XhoI revealed distinct differences between the sterile lines, and six subtypes of the S cytoplasm could be distinguished. Differences between the sterile lines were confirmed by hybridization with a $$\overline{\overline {COX}} II$$ gene probe revealing minor, line-specific hybridization signals. The data presented provide evidence for the existence of considerable variation within the only commercially used source of cms in the sugar beet, the Owen's type of cytoplasm.
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  • 3
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Cytoplasmic male sterility ; mtDNA probes ; Miniassays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Methods are described whereby hybridization of mitochondrial (mt) DNA with different DNA probes can definitely distinguish male-fertile and and male-sterile (cms) cytoplasms of sugar beet Beta vulgaris L. We have developed two types of miniassays. (1) Comparative methods requiring the isolation and restriction of total cellular DNA, hybridization with cloned mtDNA fragments from either fertile or male-sterile cytoplasms, and comparison of the hybridization patterns to the fertile-and sterile-specific patterns of mtDNA of sugar beet for the given mtDNA probe. For these analyses, we routinely used 1 g of plant material to determine the type of cytoplasm. (2) Noncomparative (“plus-minus”) methods requiring neither the isolation of pure DNA nor restriction, electrophoresis, or Southern blotting. Instead, alkaline-SDS plant extracts from as little as 50 mg of plant material were dot-blotted and hybridized with fertile-specific (mitochondrial minicircular DNA) and/or cms-specific probes (consisting of a 2.3-kb mtDNA sequence exclusively occurring in the cms cytoplasm). The assays are simple to perform, give definitive results, are nonde-structive to the plants, and may be used in mass screening of sugar beet populations for hybrid production or in in vitro culture processes.
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  • 4
    ISSN: 1432-2242
    Keywords: Primary trisomies ; Beet ; Beta vulgaris ; Isozyme polymorphism ; Chromosomal assignment ; Distorted segregation ; Dosage shift
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Segregating families of beet (Beta vulgaris) were used to verify the monofactorial inheritance of two enzyme-coding loci, leucine aminopeptidase (Lap1) and glutamate oxaloacetate transaminase (Got3). With a series of primary trisomies and using three methods to discriminate between the critical trisomic (the locus is situated on the triplicated chromosome) and the non-critical ones, it was possible to allocate the two loci to beet chromosomes I and II, respectively. For the locus Lap1 distorted segregation ratios were estimated, and the incorporation of three alleles into one plant was attempted. In the case of Got3 the measurement of the allele dosage effect after electrophoresis was chosen as the major strategy. The output of laser densitometric scans were subjected to the non-parametrical Wilcoxon-Mann-Whitney test.
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  • 5
    ISSN: 1573-5060
    Keywords: Beta vulgaris ; Beta patellaris ; Agrobacterium rhizogenes ; beet cyst nematode ; Heterodera schachtii ; nematode resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Hairy roots, induced by Agrobacterium rhizogenes, were obtained of nematode susceptible beet plants (Beta vulgaris) and of the nematode resistant alien monotelosomic addition AN5, carrying a telosome from B. patellaris. The additional telosome was found to be stably present in vitro in the roots of AN5. The hairy root cultures were inoculated with larvae of the beet cyst nematode Heterodera schachtii. On the root culture of AN5 significantly less cysts developed than on the other root cultures. These results indicate that the resistance to the beet cyst nematode is expressed in the roots after transformation and can be monitored under in vitro conditions.
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  • 6
    ISSN: 1573-5060
    Keywords: breeding ; Beta vulgaris ; sugar beet ; natural variation ; wild Beta species ; disease resistances
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Sugar beet is a relatively young crop, which supposedly has a narrow genetic base. Natural variation occurring in primitive beet varieties and in wild Beta species has been used for breeding sugar beet. This paper reviews information on desirable characteristics in Beta germplasm and the attempts made for the introgression of such characters into commercial breeding material. After an introduction on the availability of germplasm and the possibilities of hybridisation, attention is focussed on the mating system (especially male sterility), on morphological and physiological characteristics, including yield and sugar content, and on resistances to diseases and pests.
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  • 7
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: High temperature behaviour of materials for gas turbine blades manufactured in new technologyOn gas turbine blades for the middle and last stages of stationary gas turbines, the service-relevant material properties were examined. The specimens were taken from forged blades of alloys Nimonic 101 and Udimet 720 and from two fine grain versions of cast blades of alloy IN-792. In the range of service relevant temperatures long-term creep rupture tests, annealing tests, relaxations tests as well as low and high cycle fatigue tests were carried out.
    Notes: An in neuer Technologie gefertigten Schaufeln für die mittleren bis hinteren Laufreihen von stationären Gasturbinen wurden betriebsrelevante Werkstoffeigenschaften bestimmt. Die Untersuchungen erfolgten an den Schmiedelegierungen Nimonic 101 and Udimet 720 und an zwei Feinkornvarianten der Gußlegierung IN-792. Im Bereich der Anwendungstemperaturen wurden Langzeitstandversuche, Glühversuche, Relaxationsversuche sowie nieder- und hochfrequente zyklische Versuche durchgeführt.
    Additional Material: 13 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 24 (1993), S. 86-90 
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Ziel der Untersuchungen war die Analyse der Schichtwachstumsprozesse bei der plasmaunterstützten CVD-Abscheidung (PACVD) von Titannitridschichten auf Stählen im Niedertemperaturbereich ≤ 500°C.Im Vordergrund standen werkstoffkundliche Untersuchungen der verschiedenen Schichtwachstumsstadien, die sich als Funktion der Beschichtungsdauer ergeben. Die Herstellung der Schichten erfolgte in einer Puls-Plasma-CVD-Anlage. Um den gesamten Wachstumsprozeß vom Beginn der Abscheidung bis zum Wachstum geschlossener Schichten zu untersuchen, wurden die Proben bei konstanten Abscheidungsbedingungen jeweils unterschiedlich lange dem Reaktionsgasfluß ausgesetzt. Der Abbruch des Beschichtungsprozesses für die entsprechende Probe nach einer festgelegten Beschichtungszeit wurde durch die Verwendung eines Probenwechslers gewährleistet.Die Bildung der TiN-Schichten wurde an Proben aus dem Schnellarbeitsstahl S 6-5-2 (DIN 1.3343) im vergüteten Zustand untersucht. Es wurde festgestellt, daß bei diesem Substratwerkstoff zwei Oberflächenqualitäten mit unterschiedlich guten Anlagerungsbedingungen für, während der Vorbehandlung entstehende, adhäsive Verbindungen vorliegen. Die Bildung von Keimen und das Wachstum von Agglomeraten wird wesentlich durch diese Sorptionsschichten beeinflußt. Die Zahl der gebildeten Agglomerate ist bei Proben, die einer Vorbehandlung im Plasma ausgesetzt wurden, um den Faktor 10 größer als bei Proben, die während dieser Vorbehandlung abgedeckt waren. Es wurden neue Modellvorstellungen für die Entstehung und das Wachstum von Schichten entwickelt.
    Additional Material: 15 Ill.
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  • 9
    ISSN: 0935-6304
    Keywords: Capillary gas chromatography ; Mass spectrometry ; Food analysis ; Polycyclic aromatic hydrocarbons ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Twenty eight native vegetable oils were analyzed for polycyclic aromatic hydrocarbons. Generally PAH concentrations were low, but olive oils showed significantly higher contamination by light PAH ranging from 53 to 105.6 μg/kg. Muscle and liver samples of bream from the river Elbe contained little or no detectable amounts of PAH, whereas fresh and canned oysters and mussels showed higher contaminations. Highest PAH concentrations have been determined in “smoked oysters in oil” with 75.8 μg/kg benzo[a] pyrene for the oil and 12.2 μg/kg for the oyster meat.
    Additional Material: 7 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 13 (1990), S. 447-450 
    ISSN: 0935-6304
    Keywords: Capillary GC ; Atomic emission ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
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