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  • 1
    Electronic Resource
    Electronic Resource
    Selection of monosomic addition plants in offspring families using repetitive DNA probes in Beta L. (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 891-897 
    Details
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Beta patellaris ; Beta procumbens ; Monosomic additions ; PCR ; Repetitive probe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution of two repetitive DNA probes Sat-121 and PB6-4, specific for the section Procumbentes of the genus Beta, was tested in 16 B. patellaris monosomic addition families using a dot-blot hybridization procedure. All monosomic additions were accurately distinguished from diploid sib plants with both DNA probes. The probe PB6-4, with the strongest signal after hybridization, was selected for rapid screening of an extensive number of putative monosomic additions in B. patellaris or B. procumbens addition families using a squash-blot hybridization procedure. The probe PB6-4 detected 118 monosomic additions in 640 plants (18.4%) in eight different B. procumbens addition families. The addition family with chromosome 4 of B. procumbens was semi-lethal and could not be tested. The distribution of PB6-4 in B. patellaris addition families was confirmed in 63 addition families using the squash-blot procedure. In 4580 plants of these addition families, 628 individual monosomic additions (13.7%) were found. The relationship of the morphological characteristics of monosomic addition plants to the results of the squash-blot hybridization (plants with signal) using probe PB6-4 is quite rigorous but not complete. The correlation between plants with a signal and chromosome number (2n=19) is complete. These results indicate that sequences present on PB6-4 are probably present on all chromosomes of B. patellaris and B. procumbens. The possibility of utilizing the sequence information of Sat-121 for a PCR-based assay to screen for putative monosomic addition plants was also investigated as an alternative to chromosome counting. The DNA-amplification profiles using the primers REP and REP.INV clearly distinguished monosomic addition plants from their diploid sibs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221903
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  • 2
    Electronic Resource
    Electronic Resource
    Self-incompatibility reactions in wild species of the genusBeta and their relation to taxonomical classification and geographical origin (1995)
    Springer
    Genetic resources and crop evolution 42 (1995), S. 293-301 
    Details
    ISSN: 1573-5109
    Keywords: Beta ; beet ; self-incompatibility ; pollen rejection ; pollen tube growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The expression of self-incompatibility (SI) rejection patterns in 29 accessions of wildBeta species was studied at the anatomical level. Three different SI-related pollen tube rejection patterns were observed: (1) The pollen germinate from many pores and the pollen tubes are very short, often with swollen tips. (2) The pollen germinate and grow along the stigmatic papillae, where the self-incompatibility response takes place at the basis of the stigmatic papillae. (3) The pollen germinate and the pollen tubes grow with a distinct direction towards the style; the self-incompatibility response is observed at the border between the stigma and the style. In general the patterns observed inB. vulgaris L. subsp.vulgaris sensu lato seem to be in accordance with an ongoing microevolutionary process that has given rise to the largemaritima complex (viz.B. trojana Pamukçuoğlu,B. atriplicifolia Rouy, andB. maritima (L.) Arcangeli), in which it seems that the variation is related to geographical origin. The data support the idea that the endemicB. vulgaris subsp.adanensis (Pamukçuoğlu) Ford-Lloyd & Williams forms a distinct, self-compatible group separated from themaritima complex and also thatB. macrocarpa Gussone should be retained as a separate self-compatible species. For the sectionCorollinae Ulbrich the rejection patterns are in accordance with a division into two subsections (viz.,Lomatogonae andTrigynae) previously suggested on the basis of phenotypical and molecular data. The results fromProcumbentes Ulbrich are interpreted as evidence forB. procumbens Smith andB. webbiana Moquin being more related to each other than toB. patellaris Moquin, reflecting also a similar taxonomic relationship.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02432133
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