ISSN:
1618-2650
Keywords:
Best. von Cortisol in Serum
;
Radioimmunoassay
;
automatisch
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Conclusions A fully automatable method for the cortisol RIA has been developed which requires no prior extraction using an antibody in an assay-system at pH 4.0. The use of 125I-labelled tracer has abolished the need for scintillation fluid and its attendant quench and radioactive waste problems. The capacity of this method is very high because of the automation. The first clinlinal results have shown that this method is reliable, reproducible and gives comparable values with those from the 3H-assay. The fact that no centrifugation is needed, that 3 samples are counted simultaneously and that the capacity is over 1000 tubes per working day makes this method attractive to a routine laboratory with high through put of serum cortisol samples. From the theoretical stand point, this method is adaptable to almost any commercially available automatlin or semi-automatlin assay system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00482324
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