Publication Date:
2024-04-08
Description:
The Botryosphaeriales (Dothideomycetes) includes numerous endophytic, saprobic, and plant pathogenic
species associated with a wide range of symptoms, most commonly on woody plants. In a recent phylogenetic
treatment of 499 isolates in the culture collection (CBS) of the Westerdijk Institute, we evaluated the families and
genera accommodated in this order of important fungi. The present study presents multigene phylogenetic analyses
for an additional 230 isolates, using ITS, tef1, tub2, LSU and rpb2 loci, in combination with morphological data.
Based on these data, 58 species are reduced to synonymy, and eight novel species are described. They include
Diplodia afrocarpi (Afrocarpus, South Africa), Dothiorella diospyricola (Diospyros, South Africa), Lasiodiplodia
acaciae (Acacia, Indonesia), Neofusicoccum podocarpi (Podocarpus, South Africa), N. rapaneae (Rapanea,
South Africa), Phaeobotryon ulmi (Ulmus, Germany), Saccharata grevilleae (Grevillea, Australia) and S. hakeiphila
(Hakea, Australia). The results have clarified the identity of numerous isolates that lacked Latin binomials or had
been deposited under incorrect names in the CBS collection in the past. They also provide a solid foundation for
more in-depth future studies on taxa in the order. Sequences of the tef1, tub2 and rpb2 genes proved to be the
most reliable markers. At the species level, results showed that the most informative genes were inconsistent, but
that a combination of four candidate barcodes (ITS, tef1, tub2 and rpb2) provided reliable resolution. Furthermore,
given the large number of additional isolates included in this study, and newly generated multigene DNA datasets,
several species could also be reduced to synonymy. The study illustrates the value of reassessing the identity of
older collections in culture collections utilising modern taxonomic frameworks and methods.
Keywords:
Ecology
;
Evolution
;
Behavior and Systematics
;
canker and leaf spot pathogens
;
Multi-Locus Sequence Typing (MLST)
;
new taxa
;
systematics
Repository Name:
National Museum of Natural History, Netherlands
Type:
info:eu-repo/semantics/article
Format:
application/pdf
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