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  • 1
    ISSN: 1573-8248
    Keywords: Beauveria bassiana ; marcescent process ; mass production ; fermenter ; dry mycelium ; conidiation ; Beauveria bassiana ; production de masse ; fermenteur ; mycélium sec ; production de conidies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé Le mycélium deBeauveria bassiana peut être développé en milieu liquide, filtré et le mycélium séché. Après réhydratation, le mycélium sporule. Deux sources d'hydrates de carbone (sucrose et maltose) et une source azote/vitamine (extrait de levure) furent essayées pour la croissance du mycélium et la production consécutive de conidies. La croissance maximale du mycélium (12,31 mg/ml) en culture liquide, intervenait dans un milieu: sucrose (3,5%)/extrait de levure (3,5%), mais le mycélium issu d'un milieu maltose (2%)/extrait de levure (0.75%) produisait le maximum de 4,62×106 conidies/mg de mycélium sec après incubation en boîtes humides de Pétri. En utilisant les données sur la production de mycélium (en culture liquide) et sur celle de conidies (par le mycélium sec), on a calculé que les milieux sucrose (3,5%)/extrait de levure (3,5%) et maltose (2%)/extrait de levure (0,75%) produisaient la plus grande quantité de conidies par volume de milieu (l'équivalent de 3,52–3,72×107 conidies/ml).
    Notes: Abstract Mycelium ofBeauveria bassiana can be grown in liquid culture, filtered, and the mycelium dried. After rehydration the mycelium sporulates. Two carbohydrate sources (sucrose and maltose), and one nitrogen/vitamin source (yeast extract) were tested for mycelium growth and subsequent conidial production. Maximum mycelium growth (12.31 mg/ml), in liquid culture, was in the sucrose (3.5%)/yeast extract (3.5%) medium, but mycelium from a maltose (2%)/yeast extract (0.75%) medium produced the maximum of 4.62×106 conidia/mg dry mycelium after incubation in moist Petri dishes. Using the data on mycelium yield (in liquid culture) and conidial production (by dry mycelium) it is calculated that the sucrose (3.5%)/yeast extract (3.5%) and the maltose (2%)/yeast extract (0.75%) media produce most conidia per media volume (an equivalent of 3.52–3.72×107 conidia/ml).
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  • 2
    ISSN: 1573-0832
    Keywords: Beauveria bassiana ; entomopathogenic fungus ; gut infection ; histopathology ; Leptinotarsa decemlineata ; pathogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A study ofper os inoculated larvae was undertaken to determine if the entomopathogenic fungus,Beauveria bassiana, is able to infect the Colorado potato beetle,Leptinotarsa decemlineata, via the alimentary tract. Surface orper os inoculated larvae which were immediately surface sterilized post inoculation did not succumb to infection, whereas those larvae not sterilized became infected. Histological studies of fed or starved, agnotobiotic (with microbial flora) and axenic larvae revealed that conidia can germinate in the gut regardless of the presence of gut microflora. However, infection via the alimentary tract was never observed in fed larvae and only noted in a single starved individual. It is concluded that infections ofper os inoclated larvae occurred after surface contamination of the integument by viable conidia contained in the frass. The rate of food passage through the gut is probably important in preventingper os infections.
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