Publication Date:
2010-10-01
Description:
In eukaryotes, U1 small nuclear ribonucleoprotein (snRNP) forms spliceosomes in equal stoichiometry with U2, U4, U5 and U6 snRNPs; however, its abundance in human far exceeds that of the other snRNPs. Here we used antisense morpholino oligonucleotide to U1 snRNA to achieve functional U1 snRNP knockdown in HeLa cells, and identified accumulated unspliced pre-mRNAs by genomic tiling microarrays. In addition to inhibiting splicing, U1 snRNP knockdown caused premature cleavage and polyadenylation in numerous pre-mRNAs at cryptic polyadenylation signals, frequently in introns near (〈5 kilobases) the start of the transcript. This did not occur when splicing was inhibited with U2 snRNA antisense morpholino oligonucleotide or the U2-snRNP-inactivating drug spliceostatin A unless U1 antisense morpholino oligonucleotide was also included. We further show that U1 snRNA-pre-mRNA base pairing was required to suppress premature cleavage and polyadenylation from nearby cryptic polyadenylation signals located in introns. These findings reveal a critical splicing-independent function for U1 snRNP in protecting the transcriptome, which we propose explains its overabundance.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996489/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996489/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kaida, Daisuke -- Berg, Michael G -- Younis, Ihab -- Kasim, Mumtaz -- Singh, Larry N -- Wan, Lili -- Dreyfuss, Gideon -- Howard Hughes Medical Institute/ -- England -- Nature. 2010 Dec 2;468(7324):664-8. doi: 10.1038/nature09479. Epub 2010 Sep 29.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6148, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20881964" target="_blank"〉PubMed〈/a〉
Keywords:
Base Pairing
;
Base Sequence
;
HeLa Cells
;
Humans
;
Introns/genetics
;
Molecular Sequence Data
;
Oligonucleotide Array Sequence Analysis
;
Oligonucleotides, Antisense/genetics/metabolism/pharmacology
;
*Polyadenylation/drug effects/genetics
;
Pyrans/pharmacology
;
RNA Precursors/genetics/*metabolism
;
*RNA Splicing/drug effects/genetics
;
RNA, Small Nuclear/genetics/metabolism
;
Ribonucleoprotein, U1 Small Nuclear/antagonists & inhibitors/genetics/*metabolism
;
Spiro Compounds/pharmacology
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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