ISSN:
1432-072X
Keywords:
Lactic acid bacteria
;
Lactobacillus casei
;
Bacteriocin
;
Extra- and intracellular polypeptides
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract When grown in complex or synthetic media, Lactobacillus casei B 80 synthesizes a mitomycin C-inducible polypeptide with very specific bactericidal activity against the sensitive strain Lactobacillus casei B 109. The amount of secreted bacteriocin in the culture solution was low, about 1 mg/l. The bacteriocin which we called caseicin 80, was also detectable in cell extracts, although only 2% of the total activity was retained intracellularly. Caseicin 80 was concentrated by ultrafiltration and purified by cation exchange chromatography with Cellulose SE-23 and Superose. The molecular weight was in the range of M r=40,000–42,000 and the isoelectric point was pH 4.5.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00248963
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