ISSN:
1617-4623
Keywords:
Linear DNA
;
Electroporation
;
Transfection
;
DNA terminal protein
;
Bacteriophage PRD1
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Using electroporation with the phage PRD1 genome, we set up a high-frequency DNA transfer system for a linear dsDNA molecule with 5′-covalently linked terminal proteins. The transfer was saturated when more than 100 ng of PRD1 genome was used. Electroporation efficiency was about four orders of magnitude higher than that obtained with transfection. Removal of the terminal protein abolished plaque formation, which could not be rescued by supplying the terminal protein or phage DNA polymerase or both in trans.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00282449
Permalink