Publication Date:
2003-05-31
Description:
Helicobacter pylori translocates the protein CagA into gastric epithelial cells and has been linked to peptic ulcer disease and gastric carcinoma. We show that injected CagA associates with the epithelial tight-junction scaffolding protein ZO-1 and the transmembrane protein junctional adhesion molecule, causing an ectopic assembly of tight-junction components at sites of bacterial attachment, and altering the composition and function of the apical-junctional complex. Long-term CagA delivery to polarized epithelia caused a disruption of the epithelial barrier function and dysplastic alterations in epithelial cell morphology. CagA appears to target H. pylori to host cell intercellular junctions and to disrupt junction-mediated functions.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369828/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369828/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Amieva, Manuel R -- Vogelmann, Roger -- Covacci, Antonello -- Tompkins, Lucy S -- Nelson, W James -- Falkow, Stanley -- AI38459/AI/NIAID NIH HHS/ -- CA92229/CA/NCI NIH HHS/ -- DDC DK56339/DC/NIDCD NIH HHS/ -- R01 GM035527/GM/NIGMS NIH HHS/ -- R01GM35227/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2003 May 30;300(5624):1430-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA. amieva@stanford.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12775840" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens, Bacterial/genetics/*metabolism
;
Bacterial Adhesion
;
Bacterial Proteins/genetics/*metabolism
;
Cell Adhesion Molecules/metabolism
;
Cell Line
;
Cell Polarity
;
Cell Size
;
Dogs
;
Epithelial Cells/cytology/metabolism/*microbiology/ultrastructure
;
Gastric Mucosa
;
Helicobacter pylori/*pathogenicity/physiology
;
Humans
;
Intracellular Signaling Peptides and Proteins
;
Junctional Adhesion Molecules
;
Membrane Proteins/metabolism
;
Phosphoproteins/metabolism
;
Phosphorylation
;
Protein Tyrosine Phosphatase, Non-Receptor Type 11
;
Protein Tyrosine Phosphatases/metabolism
;
Tight Junctions/*microbiology/physiology/*ultrastructure
;
Tumor Cells, Cultured
;
Zonula Occludens-1 Protein
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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