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  • 1
    Electronic Resource
    Electronic Resource
    An inwardly rectifying chloride channel in ragweed-sensitized canine tracheal epithelial cells (1991)
    Springer
    European biophysics journal 20 (1991), S. 65-69 
    Details
    ISSN: 1432-1017
    Keywords: Airway epithelium ; Asthma ; Apical membrane ; Ion channel kinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The single channel inside-out patch clamp technique was used to characterize ion channels in the apical membranes of ragweed-sensitized and control canine tracheal epithelial cells maintained in primary culture. Patches were obtained from single isolated cells or from cells at the edges of confluent sheets. A new type of chloride channel was seen in sensitized cells but not in control cells. The channel showed inward rectification in symmetric chloride solutions with conductance varying from 95 pS to 52 pS over the range of −60 mV to 60 mV membrane potential. Channel gating was voltage dependent with maximal opening at about −30 mV Kinetic analysis showed that distributions of closed and open times could both be well fitted by the sums of three exponential components. Rate constants for transitions between the states of a linear kinetic model were calculated, with only one rate being significantly voltage dependent. The possible significance of this channel is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00186254
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  • 2
    Electronic Resource
    Electronic Resource
    The rhizosphere effect of tea on soil microbes in a Himalayan monsoonal location (1996)
    Springer
    Biology and fertility of soils 21 (1996), S. 131-137 
    Details
    ISSN: 1432-0789
    Keywords: Key words Rhizosphere ; Non-rhizosphere ; R:S ratio ; Tea ; Colony-forming unit ; Actinomycetes ; Bacteria ; Fungi ; Camellia sinensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Monthly investigations of the microbial population associated with tea soils, in terms of colony-forming units assessed by the plate-count method, were carried out at three different soil depths for a period of 12 months. Three groups of microbes, bacteria, actinomycetes, and fungi, were examined. Contrary to general observations, the rhizosphere:soil ratios were found to be consistently below 1 in samples taken from established tea bushes, indicating an overall negative rhizosphere effect. Interactions among certain microorganisms may also have contributed to this effect. Nevertheless, the rhizosphere of young tea plants and that of a number of other perennial plants, of different ages, growing in established tea fields, appeared to stimulate microbial growth. The negative effect of the rhizosphere of older tea bushes does not appear to be a common phenomenon that is related to the aging of plants in general, but seems to be unique and specific to tea plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335924
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