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  • 1
    Digitale Medien
    Digitale Medien
    Isolation and expression analysis of two yeast regulatory genes involved in the derepression of glucose-repressible enzymes (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 366-373 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Glucose repression ; Glucose derepression ; Regulatory genes ; Expression analysis ; Saccharomyces cerevisiae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Yeast strains carrying one of the two regulatory mutations cat1 and cat3 are defectve in derepression of several glucose-repressible enzymes that are necessary for utilizing non-fermentable carbon sources. Hence, these strains fail to grow on ethanol, glycerol or acetate. The synthesis of isocitrate lyase, malate synthase, malate dehydrogenase and fructose-1,6-bisphosphatase is strongly affected in cat1 and cat3 strains. Genes CAT1 and CAT3 have been isolated by complementation of the cognate, mutations after transformation with an episomal plasmid gene library. The restriction map of CAT1 proved its allelism to the earlier isolated SNF1 gene. Both genes appear to exist as single-copy genes per haploid genome as indicated by Southern hybridization. Northern analysis has shown that the 1.35 kb CAT3 mRNA is constitutively expressed, independent of the carbon source in the medium. Derepression studies with CAT3 transformants using a multi-copy plasmid showed over-expression of glyoxylate cycle enzymes. This result would be consistent with a direct effector function for the CAT3 gene product.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00329667
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Constitutive and carbon source-responsive promoter elements are involved in the regulated expression of the Saccharomyces cerevisiae malate synthase gene MLS1 (1997)
    Springer
    Molecular genetics and genomics 255 (1997), S. 619-627 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Key wordsSaccharomyces cerevisiae ; Gluconeogenesis ; Malate synthase ; Transcriptional regulation ; MLS1
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The malate synthase gene, MLS1, of the yeast Saccharomyces cerevisiae is transcriptionally regulated by the carbon source in the growth medium. A MLS1-lacZ fusion gene, expressed at a basal level in the presence of 2% glucose, is derepressed more than 100-fold under conditions of sugar limitation. No evidence for MLS1 induction by oleic acid was found. By deletion analysis of the MLS1 control region, we identified two sites, UAS1 and UAS2, as important for efficient derepression of the gene. Both sites contain sequences that resemble the previously characterized carbon source-responsive element (CSRE) found in the promoter of the isocitrate lyase gene ICL1. Indeed, UAS1 and UAS2 in the MLS1 upstream region turn out to be functional CSRE sequence variants. This finding allowed us to define a modified version of the CSRE consensus sequence (CCRTYSRNCCG). Protein binding to UAS1MLS1 was observed with extracts from derepressed but not from repressed cells, and could be competed for by an excess of the unlabelled CSRE(ICL1) sequence. No competition was observed with a mutated CSRE variant. Site-directed mutagenesis of both CSREs in the MLS1 promoter reduced gene activation under derepressing conditions to 20% of the wild-type level. The same decrease was observed with the wild-type MLS1 promoter in a cat8 mutant, lacking an activator of CSRE-dependent transcription. The CSRE/Cat8p-independent activation of MLS1 is mediated by constitutive UAS elements. The pleiotropic transcription factor Abf1p, which binds to the MLS1 upstream region, may contribute to constitutive activation. Thus, in order to ensure the severe glucose repression of MLS1 observed, repressor elements that respond to the carbon source must counteract constitutive activation. In summary, ICL1 and MLS1 share common cis-acting elements, although a distinct mechanism of carbon source control also contributes to MLS1 regulation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004380050536
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Fabrication of glassy carbon microstructures by pyrolysis of microfabricated polymeric precursorsThis work has been supported in part by the Office of Naval Research and the Defense Advanced Research Projects Agency. It used MRSEC Shared Facilities supported by the National Science Foundation (DMR-9400396). The authors thank Xiao-Mei Zhao for demonstrating microtransfer molding. (1997)
    Weinheim : Wiley-Blackwell
    Advanced Materials 9 (1997), S. 477-480 
    Details
    ISSN: 0935-9648
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/adma.19970090604
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Bausteine von Oligosacchariden, LXXVIII. Synthese von KDO-haltigen Lipoid-A-Analoga (1987)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1987 (1987), S. 249-258 
    Details
    ISSN: 0170-2041
    Schlagwort(e): Chemistry ; Organic Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Building Units of Oligosaccharides, LXXVIII. - Synthesis of KDO-Containing Lipid A AnaloguesThe non-neighbouring group supported glycosidation of 12 with the suitably protected glycosyl bromide 9 of 2-azido-2-deoxy-D-glucose leads - in the presence of a heterogeneous silver catalyst - to the formation of the β-(1→6)-glycosidically linked disaccharide 14. It consists of two 2-azido-2-deoxy-D-glucose units. Partial deblocking of 14 furnishes 15. On glycosidation with KDO bromide this compound yields the trisaccharide 16, which contains a KDO unit in an α-(2→6) ketosidic bond. Reduction of the two azido groups followed by amidation with (R)-3-hydroxymyristic acid and further deblocking generates the trisaccharide α-KDO-(2→6)-β-D-GlcA-(1→6)-D-GlcA 20 with two 3-hydroxy fatty acid residues in an amidic linkage.
    Notizen: Die Umsetzung des Pyranosylbromids 9 der 2-Azido-2-desoxy-D-glucose mit dem Akzeptor 12 führt bei Gegenwart eines heterogenen Silberkatalysators ohne Nachbargruppenbeteiligung unter Inversion zum β-(1→6)-glycosidisch verknüpften Disaccharid 14 aus zwei 2-Azido-2-desoxy-D-glucose-Einheiten. Nach partieller Entblockierung zu 15 ist die Anknüpfung eines KDO-Restes unter Bildung einer α-(2→6)-ketosidischen Bindung zum Trisaccharid 16 möglich. Nach Reduktion der Azidogruppen und Anknüpfung von (R)-3-Hydroxymyristinsäure-Resten gelangt man nach Entblockierung zum Trisaccharid α-KDO-(2→6)-β-D-GlcA-(1→6)-D-GlcA 20, das amidartig zwei 3-Hydroxyfettsäure-Reste gebunden enthält.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jlac.198719870316
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Verzweigte und kettenverlängerte Zucker, XXX. Diastereoselektive Synthese von L-glycero-D-manno-Heptose, einem Baustein der inneren Core-Region von Lipopolysacchariden (1986)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1986 (1986), S. 675-686 
    Details
    ISSN: 0170-2041
    Schlagwort(e): Chemistry ; Organic Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Branched and Chain-extended Sugars, XXX.  -  Diastereoselective Synthesis of L-glycero-D-manno-Heptose, a Constituent of the Inner Core Region of LipopolysaccharidesReaction of 2,3;5,6-di-O-isopropylidene-D-mannofuranose (1) with 2-lithio-1,3-dithiane affords diastereoselectively the 3,4;6,7-di-O-isopropylidene-D-glycero-D-galacto-heptose trimethylene dithioacetal (3). Conversion of compound 3 by a sequence of steps gives the tri-O-isopropylidene-D-glycero-D-galacto-heptit 16 which is oxidized with 1,1′-(azodicarbonyl)-dipiperidine to give the tri-O-isopropylidene-L-glycero-D-manno-heptose 17. Finally, compound 17 is transferred via the acetate 19 into the L-glycero-D-manno-heptopyranose 18.
    Notizen: 2,3;5,6-Di-O-isopropyliden-D-mannofuranose (1) reagiert diastereoselektiv mit 2-Lithio-1,3-dithian zum 3,4;6,7-Di-O-isopropyliden-D-glycero-D-galacto-heptose-trimethylendithioacetal (3). Nach Umwandlung von 3 über eine Reihe von Zwischenstufen zum Tri-O-isopropyliden-D-glycero-D-galacto-heptit 16 läßt sich dieser mit 1,1′-(Azodicarbonyl)dipiperidin zur Tri-O-isopropyliden-L-glycero-D-manno-heptose 17 oxidieren. Hieraus ist über das Acetat 19 die freie L-glycero-D-manno-heptopyranose 18 zu gewinnen.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jlac.198619860409
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  • 6
    facet.materialart.
    Unbekannt
    Crustal and uppermost mantle velocity structure of northern Eurasia along the profile Quartz (1997)
    In:  Bull. Seism. Soc. Am., Wiesbaden, Franz Steiner Verlag GmbH, vol. 87, no. 2, pp. 414-426, pp. L10308, (ISSN: 1340-4202)
    Details
    Publikationsdatum: 1997
    Schlagwort(e): Earth model, also for more shallow analyses ! ; Velocity depth profile ; EUROPROBE (Geol. and Geophys. in eastern Europe) ; Deep seismic sounding (espec. cont. crust) ; BSSA
    Standort Signatur Erwartet Verfügbarkeit
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    BIBLIOGRAFIE SEISMOLOGIE
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