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  • 1
    ISSN: 1432-0878
    Keywords: Avian median eminence ; Noradrenergic innervation ; Microspectrofluorimetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The median eminence of duck, following freeze-drying and formaldehyde treatment (method of Falck-Hillarp), revealed within the subependymal layer of the internal zone a plexus of green fluorescent nerve fibers that encircled scattered neurons which apparently belong to the basal infundibular (tuberal) nucleus. Scarce fluorescent structures accompanied by a diffuse weak fluorescent reaction were seen in the reticular and palisade layers of the external zone. Fluorescent sympathetic fibers surrounded primary portal vessels in the perivascular space. Microspectrofluorimetric analysis revealed the presence of catecholamines in all fibers measured. After HCl treatment, the modifications of the excitation spectra indicated that the fibers in the subependymal layer of the inner median eminence contained noradrenaline. Dopaminergic fibers were not found in this region. The fluorescent contents of noradrenergic fibers was depleted by reserpine. After inhibition of dopamine-β-hydroxylase by FLA 63, some dopamine could be demonstrated in these noradrenergic fibers. After three successive intraventricular injections of 6-OH-dopamine their fluorescent contents disappeared. However, three months after this treatment some green fluorescent fibers were again seen in this location.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 143 (1973), S. 143-147 
    ISSN: 1432-0878
    Keywords: Rat ; Suprachiasmatic nucleus ; Blinding ; Retinohypothalamic connections ; Karyometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Radioaktiv markierte Aminosäuren werden bei Säugetieren nach intraokulärer Injektion nicht nur zu den primären optischen Kerngebieten, sondern auch in den Nucleus suprachiasmaticus des Hypothalamus transportiert (Moore und Lenn, 1972; Hendrickson, Wagoner und Cowan, 1972; Moore, 1973). Dieser Befund macht eine direkte Verknüpfung zwischen der Netzhaut und dem sekretorisch aktiven Nucleus suprachiasmaticus wahrscheinlich, obwohl mit Silbertechniken eine solche Verbindung nicht sicher nachgewiesen werden konnte. Karyometrische Studien am Nucleus suprachiasmaticus männlicher Wistar-Ratten zeigen 6 Tage nach beidseitiger Blendung eine signifikante Abnahme der Zellkerndurchmesser. Dieses Ergebnis spricht für eine Aktivitätsänderung der Neurone im Nucleus suprachiasmaticus geblendeter Ratten.
    Notes: Summary Intraocular injection of labeled amino acids results, in different mammals, in an accumulation of radioactive material not only in the primary optic centers but also within the hypothalamic suprachiasmatic nucleus (Moore et al., 1972; Hendrickson et al., 1972; Moore, 1973). This finding argues in favour of a direct connection between the retina and the secretory suprachiasmatic nucleus although silver techniques do not show such a pathway. In male Wistar rats the nuclear diameter of the suprachiasmatic neurons decreases significantly 6 days after bilateral experimental ablation of the retina. These results indicate an alteration in activity of the suprachiasmatic neurons in blinded rats.
    Type of Medium: Electronic Resource
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