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  • Organic Chemistry  (3)
  • Auxotrophs  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 75 (1988), S. 869-874 
    ISSN: 1432-2242
    Keywords: Auxin ; Auxotrophs ; BUdR ; Enrichment ; Temperature sensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A protocol has been developed for the negative selection of plant auxotrophs using the nucleoside analogues BUdR and FUdR. The protocol was optimised using nitrogen-starved protoplast-derived cells of Nicotiana plumbaginifolia to simulate auxotrophy. The present results represent a significant improvement over previous reports in that: 1) The background of colonies escaping BUdR/FUdR kill is low and reproducible. 2) The protocol was improved to the point where background survival was 0.03% for non-starved cultures and 0.09% for auxin-starved cultures. 3) It was shown that UV irradiation decreases BUdR sensitivity of dividing cells and that this is overcome by increased exposure to BUdR. 4) Application of the method to auxin-starved haploid protoplast-derived cell suspensions resulted, for the first time, in the selection of temperature-sensitive (ts) auxin auxotrophs. 5) It could be demonstrated, for the first time, that the method in practice enriches for auxotrophs, in this case by a factor of 10 for auxin auxotrophs and at least 60 for ts auxin auxotrophs.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 31 (1948), S. 1617-1623 
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Durch Reduktion mit Lithiumaluminiumhydrid wurde aus den entsprechenden Estern von α-Aminocarbonsäuren die folgenden Aminoalkohole dargestellt: D, L-Phenylalaninol, L(-)-Phenylalaninol, L(-)-Tyrosinol, L(+)-Alaninol, L(+)-Leucinol, L,(+)-2-Oxymethylpyrrolidin (L(+)-Prolinol), L(+)-2-Aminobutandiol-(1,4) (L(+)-Asparaginol), D, L-2-Aminobutandiol-(1,4) (D, L-Asparaginol) und 2-Amino-propandiol-( 1,3).
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 32 (1949), S. 1156-1157 
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Durch Reduktion mit Lithiumaluminiumhydrid wurden aus L-Valin-methylester L-Valinol und aus L-Tyrosin-methylester L-Tyrosinol in guten Ausbeuten hergestellt.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 32 (1949), S. 1936-1938 
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Durch Reduktion von Dibenzoyl-L-histidinmethylester mit LiAlH4 wurden Monobenzoyl-L-histidinol und aus letzterem durch Verseifung L-Histidinol hergestellt. Letztere Verbindung zeigt ähnliche pharmakologische Wirkung wie Histamin, aber erst in 1000-3000 mal höherer Dosis.
    Type of Medium: Electronic Resource
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