ISSN:
1432-2048
Keywords:
Auxin biosynthesis
;
Cellular compartmentation
;
Hordeum (IAA biosynthesis)
;
Tryptophan
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Protoplast preparations from barley (Hordeum vulgare L.) enzymatically converted [5-3H]tryptophan to [3H]indole-3-acetic acid (IAA). Both a chloroplast and a crude cytoplasmic fraction, isolated from protoplasts that had previously been fed [5-3H]tryptophan, contained [3H]IAA. Chloroplast and cytoplasmic preparations, isolated from protoplasts and thereafter incubated with [5-3H]tryptophan, also synthesized [3H]IAA, although, in both instances the pool size was less than 50% of that detected in the in-vivo feeds. There were no significant differences in the amounts of [3H]IAA that accumulated in protoplast and chloroplast preparations incubated in light and darkness.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00392778
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