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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of phospholipase A2 activation by plasmin in cultured bovine endothelial cells (1996)
    Springer
    Journal of biomedical science 3 (1996), S. 59-66 
    Details
    ISSN: 1423-0127
    Keywords: Plasmin ; Calcium influx ; Cytosolic phospholipase A2 ; Endothelial cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Treatment of cultured bovine carotid artery endothelial cells with 0.1 µM human plasmin has been reported to induce a receptor-mediated short burst of arachidonate release, which is a pertussis toxin-sensitive and extracellular calcium-dependent reaction. Plasmin-induced calcium influx in cells was significantly inhibited by pretreatment with pertussis toxin, indicating that the former was coupled with a pertussis toxin-sensitive guanosine 5′-triphosphate (GTP)-binding protein. Plasmin significantly induced the formation of lysophosphatidylcholine but not lysophosphatidylethanolamine. A cellular phospholipase A2 with an arachidonyl specificity at the sn-2 position of phosphatidylcholine, which required submicromolar calcium, was identified as a cytosolic phospholipase A2 by immunoblot analysis. By a cell-free enzyme activity assay and immunoblot analysis, plasmin was found to induce a translocation of the cytosolic phospholipase A2 from the cytosol to the membrane. Taken together, the results suggest that plasmin bound to its putative receptor and activated a GTP-binding protein coupled to calcium influx channel, followed by translocation and activation of cytosolic phospholipase A2 in endothelial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02253580
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  • 2
    Electronic Resource
    Electronic Resource
    Theoretical study on the reaction mechanism of the alcoholysis of isocyanates (1992)
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 42 (1992), S. 403-409 
    Details
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In this paper, the reactions of HNCO or CH3NCO with methanol have been studied using the ab initio MO method. The geometries of the reactants and products have been optimized by the energy gradient method. The calculated results are in accordance with experiment. Using Powell's method, by the minimization of the Euclidean norm σ of the gradient, the structures of the transition state (TS) for the two reactions were obtained. The structures have been further confirmed as TS by finding that there is one and only one negative eigenvalue for their force constant matrix. The imaginary vibration mode corresponding to the TS was also discussed. The calculated activation barriers of these two reactions are 96.02 and 95.13 kJ/mol, respectively. It can be concluded that the alcoholysis reaction of isocyanate is a nucleophilic addition reaction with methanol as an electronic donor and isocyanate as an acceptor and that the hydroxyl hydrogen of CH3OH plays an important role in the reaction.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/qua.560420303
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