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  • Protoplast vacuole  (2)
  • Atomabsorption, flammenlos  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Energy-dependent uptake of malate into vacuoles isolated from barley mesophyll protoplasts
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Bioenergetics 806 (1985), S. 311-319 
    Details
    ISSN: 0005-2728
    Keywords: (Barley) ; Energy dependence ; Malate uptake ; Protoplast vacuole
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2728(85)90110-0
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  • 2
    Electronic Resource
    Electronic Resource
    Polypeptide pattern and enzymic character of vacuoles isolated from barley mesophyll protoplasts (1986)
    Springer
    Planta 169 (1986), S. 345-355 
    Details
    ISSN: 1432-2048
    Keywords: Hordeum (protoplast vacuole) ; Hydrolase ; Polypeptide ; Protoplast vacuole ; Tonoplast ; Vacuole sap
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Intact chloroplasts and vacuoles were isolated from mesophyll protoplasts of barley. The chloroplasts occupied about 15% of the cellular volume and contained 75% of the protein, whereas the vacuoles occupied about 80% of the volume and contained less than 4% of total cellular protein. Contamination of the vacuolar fraction by foreign protein is included in these values. Chlorophyll was absent from the vacuolar fraction, but less than 1% of several extra-vacuolar marker proteins were still present. The vacuoles contained hydrolytic enzymes. Several of them (α-mannosidase, α-galactosidase, N-acetylglucosaminidase) were soluble, whereas part of the activity of others semimented with the tonoplasts during centrifugation. Attached proteins could be released from the membranes during freezing in the presence of NaCl. One-dimensional gel electrophoretic separation of soluble vacuolar proteins under non-denaturing conditions yielded more than 10 protein bands. A comparative analysis was performed of thylakoids and vacuoles which were subfractionated into tonoplasts and soluble vacuolar constituents. Sodium dodecyl sulfate gel electrophoresis separated about 15 polypeptides of the soluble fraction which reacted with silver reagent. The tonoplast fraction yielded about 20 bands. A similar number of bands was observed when vacuoles incubated with the 14C-labelled SH-reagent N-ethylmaleimide were analysed for radioactive polypeptides. Silverstaining of the polypeptides and their SH-content did not correlate. Several polypeptides of the vacuolar fraction had molecular weights very similar to the molecular weights of known chloroplast proteins. However, with the exception of the two subunits of ribulose-1,5-bisphosphate carboxylase, contamination of the vacuolar fraction by chloroplast proteins could be ruled out as a possible cause of the close correspondence. The lipophilic carboxylic-group reagent N,N′-dicyclohexylcarbodiimide ([14C]DCCD) reacted with several polypeptides of thylakoids and tonoplasts. However, the labelling patterns were different. The most heavily labelled polypeptide of thylakoids was the 8-kDa polypeptide of the basal part of the coupling factor CF0. Tonoplast polypeptides heavily labelled with [14C]DCCD had molecular weights of 24, 28, and 56 kDa. The vacuolar 8-kDa polypeptide remained unlabelled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392130
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  • 3
    Electronic Resource
    Electronic Resource
    Untersuchung von systematischen Fehlern bei der Bestimmung von Hg-Gesamtgehalten im Bereich 〈 10−5% in anorganischen und organischen Matrices mit zwei unabhängigen Verbundverfahren (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 291 (1978), S. 278-291 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Quecksilber ; Spektrometrie, Emission/Spektralphotometrie, MIP-Technik ; Atomabsorption, flammenlos ; Kalt-Dampf-Technik ; systematische Fehler
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Systematische Fehler, die bei der Bestimmung von Hg im ng/g und pg/g-Bereich vor allem bei der Probennahme, Probenvorbereitung und beim Probenaufschluß auftreten, wurden mit 203Hg Radiotracertechniken und mit zwei für den Spurenbereich entwickelten Verbundverfahren untersucht. Beim emissionsspektrometrischen Verfahren (OES-MIP) wird die Probe im mikrowelleninduzierten Sauerstoff Plasma (MIP) verascht und das Hg nach Abtrennung mit einem Au-Absorber in einem Argon-Plasma angeregt. Das emittierte Hg-Licht wird mit einer Photodiode registriert. Nachweisgrenze: 0,01 ng Hg; Variationskoeffizient: 5% für 1 ng Hg. Beim zweiten Verfahren wird Hg nach dem Probenaufschluß (HClO3/HNO3) mit einem mechanisierten Aufschlußsystem nach der Kalt-Dampf-Technik (Ascorbinsäure/SnCl2 bzw. NaBH4) freigesetzt, an einem Gold-Absorber gesammelt und anschließend durch flammenlose AAS bei 253,7 nm bestimmt. Nachweisgrenze: 0,5 ng Hg; Variationskoeffizient: 5% für 5 ng Hg.
    Notes: Summary In the determination of Hg at ng/g and pg/g levels systematic errors are due to faults in the analytical methods such as intake, preparation and decomposition of a sample. The sources of these errors have been studied both with 203Hg-radiotracer techniques and two multi-stage procedures developed for the determination of trace levels. The emission spectrometric (OES-MIP) procedure includes incineration of the sample in a microwave induced oxygen plasma (MIP), the isolation and enrichment on a gold absorbent and its excitation in an argon plasma (MIP). The emitted Hg-radiation (253,7 nm) is evaluated photometrically with a semiconductor element. The detection limit of the OES-MIP procedure was found to be 0,01 ng, the coefficient of variation 5% for 1 ng Hg. The second procedure combines a semi-automated wet digestion method (HClO3/HNO3) with a reduction-aeration (ascorbic acid/SnCl2), and the flameless atomic absorption technique (253,7 nm). The detection limit of this procedure was found to be 0,5 ng, the coefficient of variation 5% for 5 ng Hg.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00491943
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