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  • Astrophysics  (1)
  • Bowman-Birk inhibitor  (1)
  • 1
    ISSN: 1573-4943
    Keywords: Bowman-Birk inhibitor ; microcalorimetry ; enthalpy ; fluorescence polarization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The binding of α-chymotrypsin to black-eyed pea trypsin/chymotrypsin inhibitor (BTCI) has been studied using the inhibitory activity against the enzyme and the formation of the complex enzyme/inhibitor followed by measurements of fluorescence polarization. Apparent equilibrium constants were estimated for several temperatures and the values obtained range from 0.32 × 107 to 1.36 × 107 M−1. The following values were found from van't Hoff plots: Δ H vh ° = 10.8 kcal mol-1 (from inhibitory assays) and 11.1 kcal mol−1 (from fluorescence polarization); ΔS° = 67.9 and = 67.8 kcal K−1 mol−1, respectively. Calorimetric binding enthalpy was determined (corrected for the ionization heat of the buffer) and the resulting value was ΔH cal ° = 4.9 kcal mol-1. These results indicate that the binding of chymotrypsin to BTCI is an entropically driven process.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2019-07-13
    Description: A novel laboratory experimental design is described that will investigate the processing of dust grains in astrophysical shocks. Dust is a ubiquitous ingredient in the interstellar medium (ISM) of galaxies; however, its evolutionary cycle is still poorly understood. Especially shrouded in mystery is the efficiency of grain destruction by astrophysical shocks generated by expanding supernova remnants. While the evolution of these remnants is fairly well understood, the grain destruction efficiency in these shocks is largely unknown. The experiments described herein will fill this knowledge gap by studying the dust destruction efficiencies for shock velocities in the range of approximately 10-30 kilometers per second (microns per nanosecond), at which most of the grain destruction and processing in the ISM takes place. The experiments focus on the study of grain-grain collisions by accelerating small (1 millimeter) dust particles into a large (approximately 5-10 millimeter diameter) population; this simulates the astrophysical system well in that the more numerous, small grains impact and collide with the large population. Facilities that combine the versatility of high-power optical lasers with the diagnostic capabilities of X-ray free-electron lasers, e.g., the Matter in Extreme Conditions instrument at the SLAC (originally named Stanford Linear Accelerator Center) National Accelerator Laboratory, provide an ideal laboratory environment to create and diagnose dust destruction by astrophysically relevant shocks at the micron scale.
    Keywords: Astrophysics
    Type: GSFC-E-DAA-TN67044 , High Power Laser Science and Engineering (ISSN 2095-4719) (e-ISSN 2052-3289); 6; e39
    Format: application/pdf
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