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  • 1
    ISSN: 1573-5060
    Keywords: Aflatoxin ; Arachis hypogaea ; Aspergillus flavus ; colony-incidence ; colonization density ; genotype resistance ; groundnut ; peanut ; Israel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Field and microplot trials were conducted. In microplot experiments the soil was artificially infested by Aspergillus flavus Link ex Fries. In field tests the percentage of A. flavus-affected kernels and in microplot experiments the number of A. flavus colonies emerging from 100 seeds was determined. As the geocarpoplane is the main frontier of pod invasion, pod surfaces were measured and the number of seedborne colonies per 100 cm2 of pod surface were calculated for each groundnut genotype. Some genotypes, which were similar in colony incidence (counts per 100 seeds), differed markedly from each other in colonization density (number of seedborne colonies as calculated per unit area of pod surface). Cultivar EC-21115 had a low incidence of seed borne colonies of A. flavus; however, the number of colonies per 100 cm2 of pod surface was high. Cv. Lavkan was low, both, in colony incidence and colonization density. Genotypes low in both these parameters should be tested as potential sources of resistance.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 122 (1993), S. 177-183 
    ISSN: 1573-0832
    Keywords: Aflatoxin ; Aspergillus flavus ; A. parasiticus ; Cyclopiazonic acid ; ELISA ; Groundnuts (peanuts) ; Israel ; Sterigmatocystin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Two hundred strains of the Aspergillus flavus group isolated from groundnuts (peanuts) growing in Israel were examined for their ability to produce mycotoxins in potato dextrose (PD) broth. Almost 77% of the isolates produced aflatoxin; aflatoxins B1 and B2 were formed by most of the isolates. Simultaneous production of aflatoxins of groups B and G was detected in only 0.5% of the isolates. Microscopic examination revealed that 98% of the isolates wereA. flavus and only 2%A. parasiticus. Cyclopiazonic acid (CPA) was detected in 22.5% of the isolates, including 3.5% that produced only CPA. Sterigmatocystin was detected in only 2% of the isolates and only one isolate produced aflatoxin simultaneously with CPA and sterigmatocysin. The dry weight (DW) of mycelium, 7 days after inoculating the medium, was between 71–110 mg/30 ml medium in more than 70% of the isolates. A general decrease in the pH was observed and 75% of the isolates reduced the pH to 4.5 or below. After 14 days, a small increase in DW and an increase in the pH toward neutrality was observed. On PD agar, 30% of the isolates produced sclerotia, including 5% that produced them profusely. No correlation between mycelial growth, changes in pH of the medium, sclerotium formation, and aflatoxin accumulation could be observed. The mycotoxigenic potential of theA. flavus strains isolated from groundnuts seems to be relatively high and may present a potential threat to human and animal health.
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