ISSN:
1040-0397
Keywords:
Ascorbic Acid
;
Amplification
;
Biosensor
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The current response of L-ascorbic acid at an ascorbate-oxidase membrane electrode (detecting the decrease of oxygen) has been amplified by adding dithiothreithol into the sample solution. The signal amplification is based on the cyclic reaction between L-ascorbic acid and dehydroascorbic acid, which leads to the regeneration of L-ascorbic acid. The amplification factor was increased up to 22.5 (at pH 7.5) when 5 × 10-3 M dithiothreithol solution was used. This amplification system enables the determination of not only L-ascorbic acid but dehydroascorbic acid, and the detection limit for vitamin C was found to be 2 × 10-7 M.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140050805
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