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  • Arabidopsis thaliana  (10)
  • 1
    Electronic Resource
    Electronic Resource
    Cloning and characterization of two cDNAs encoding casein kinase II catalytic subunits in Arabidopsis thaliana (1993)
    Springer
    Plant molecular biology 21 (1993), S. 279-289 
    Details
    ISSN: 1573-5028
    Keywords: casein kinase II ; Arabidopsis thaliana ; cDNA sequence ; expression in Escherichia coli ; enzyme activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two cDNA clones, ATCKA1 and ATCKA2, encoding casein kinase II (CKII) catalytic subunits, were cloned from Arabidopsis thaliana and their nucleotide sequences were determined. Both cDNAs contain 999 bp open reading frames and are 94% identical on the amino acid sequence level. The deduced amino acid sequences of ATCKA1 and ATCKA2 are very similar to that of the human CKII catalytic α subunit (72% homology). Northern blot analysis indicates that the ATCKA1 and ATCKA2 mRNAs are present in all plant organs, but that ATCKA1 transcript levels are quite low compared to those of ATCKA2. Genomic Southern blot analysis suggests that there are at least three CKII genes in the A. thaliana genome. We expressed the ATCKA1 and ATCKA2 cDNAs in Escherichia coli using a pET vector derivative and analyzed the expressed protein in vitro. The expressed ATCKA1 protein phosphorylated casein using either ATP or GTP. This activity was inhibited by heparin, indicating that the expressed protein has activity similar to those reported for animal and yeast CKII.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019944
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  • 2
    Electronic Resource
    Electronic Resource
    AtPLC2, a gene encoding phosphoinositide-specific phospholipase C, is constitutively expressed in vegetative and floral tissues in Arabidopsis thaliana (1997)
    Springer
    Plant molecular biology 34 (1997), S. 175-180 
    Details
    ISSN: 1573-5028
    Keywords: phosphoinositide-specific phospholipase C ; chromosomal mapping ; Arabidopsis thaliana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA encoding a phosphoinositide-specific phospholipase C (PI-PLC) from the higher plant Arabidopsis thaliana was cloned and characterized. The gene corresponding to this cDNA is designated AtPLC2. The overall structure of the predicted AtPLC2 protein is similar to those of plant PI-PLCs and mammalian δ-type PI-PLCs. Northern blot analysis revealed that AtPLC2 is expressed constitutively whereas AtPLC1S, another gene for PI-PLC of Arabidopsis, is induced by environmental stresses such as dehydration and salinity, indicating that the function of AtPLC2 is distinct from that of AtPLC1S. The AtPLC2 mRNA was detected in vegetative and floral tissues. We determined the positions of these two PI-PLCs genes on Arabidopsis chromosomes by RFLP mapping using P1 genomic clones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005885230896
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  • 3
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of a gene from Arabidopsis thaliana encoding a myb homologue (1992)
    Springer
    Plant molecular biology 19 (1992), S. 493-499 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; myb-related gene ; PCR ; promoter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A gene encoding a proto-oncogene, a myb-related gene named Atmyb1, was cloned from Arabidopsis thaliana, and its nucleotide sequence was determined. The Atmyb1 gene contains an intron of 494 bp, and there are no highly homologous sequences present in the A. thaliana genome, but evidence was found that other myb-related genes exist. In the 5′ flanking region, we found several typical cis-acting elements found in plant promoters. Sequence comparisons revealed that the ATMYB1 protein has a putative DNA-binding domain with two repeats of tryptophan clusters, which is common in MYB-related proteins in plants, while animal MYB-related proteins contain DNA-binding domains with three repeats of tryptophan clusters. The putative DNA-binding domain of the ATMYB1 protein has higher homology with that of the human c-MYB protein than with those of other plant MYB proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023398
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  • 4
    Electronic Resource
    Electronic Resource
    Sequencing and characterization of the kinesin-related geneskatB andkatC ofArabidopsis thaliana (1994)
    Springer
    Plant molecular biology 25 (1994), S. 865-876 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; nucleotide sequences ; kat genes ; kinesin-like proteins ; microtubule-stimulated ATPase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Complementary DNAs of two kinesin-related genes,katB andkatC, were isolated fromArabidopsis thaliana and sequenced. The carboxyl-terminal regions of the polypeptides encoded by these genes, especially the presumptive ATP-binding and microtubule-binding domains, share significant sequence homology with the mechanochemical motor domain of the kinesin heavy chain. The predicted secondary structures of KatB and KatC proteins include a large globular domain in the carboxyl-terminal region and a small globular domain in the amino-terminal region that are separated by a long α-helical coiled-coil with heptad repeats. A truncated KatC polypeptide (KatC(207–754)), which includes the carboxylterminal region of KatC, was expressed inEscherichia coli and was shown to possess microtubule-stimulated ATPase activity and to bind to microtubules in an ATP-sensitive manner, both of which are characteristics of kinesin and kinesin-like proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028881
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular cloning and characterization of a gene that encodes a MYC-related protein in Arabidopsis (1996)
    Springer
    Plant molecular biology 32 (1996), S. 571-576 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; basic/helix-loop-helix (bHLH) ; Myc-related protein ; RFLP mapping ; Sph box ; transcription factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In plants, MYC-related proteins function as transcription factors involved in anthocyanin production and trichome development. We cloned a gene, Atmyc1, and its corresponding cDNA, that encodes for a MYC-related protein from Arabidopsis thaliana. The putative protein has a basic/helix-loop-helix motif at the C-terminus and a highly homologous region with that of the maize B/R family at the N-terminus. The promoter region of Atmyc1 contains a Sph box (CATGCATG) that is known as a cis-regulatory element conferring seed-specific expression. In fact, Atmyc1 transcripts were more abundant in developing seeds than in stems and leaves where trichomes are normally expressed. Restriction fragment length polymorphism mapping demonstrated that Atmyc1 is located on the upper region of chromosome 4, which clearly indicates that Atmyc1 is distinct from the ttg (transparent testa glabrous) locus that affects both trichome development and anthocyanin biosynthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019112
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular cloning of a cDNA encoding diacylglycerol kinase (DGK) in Arabidopsis thaliana (1996)
    Springer
    Plant molecular biology 30 (1996), S. 647-653 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; diacylglycerol kinase ; EF-hand ; cysteine-rich zinc finger ; PI turnover ; Ca2+-binding proteins ; signal transduction pathways ; plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diacylglycerol kinase (DGK) synthesizes phosphatidic acid from diacylglycerol, an activator of protein kinase C (PKC), to resynthesize phosphatidylinositols. The structure of DGK has not been characterized in plants. We report the cloning of a cDNA, cATDGK1, encoding DGK from Arabidopsis thaliana. The cATDGK1 cDNA contains an open reading frame of 2184 bp, and encodes a putative protein of 728 amino acids with a predicted molecular mass of 79.4 kDa. The deduced ATDGK1 amino acid sequence exhibits significant similarity to that of rat, pig, and Drosophila DGKs. The ATDGK1 mRNA was detected in roots, shoots, and leaves. Southern blot analysis suggests that the ATDGK1 gene is a single-copy gene. The existence of DGK as well as phospholipase C suggests the existence of PKC in plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00049339
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  • 7
    Electronic Resource
    Electronic Resource
    Identification of a cis-regulatory region of a gene in Arabidopsis thaliana whose induction by dehydration is mediated by abscisic acid and requires protein synthesis (1995)
    Springer
    Molecular genetics and genomics 247 (1995), S. 391-398 
    Details
    ISSN: 1617-4623
    Keywords: Arabidopsis thaliana ; Dehydration-responsive gene ; Abscisic acid ; Promoter ; β-Glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In Arabidopsis thaliana, the induction of a dehydration-responsive gene, rd22, is mediated by abscisic acid (ABA) but the gene does not include any sequence corresponding to the consensus ABA-responsive element (ABRE), RYACGTGGYR, in its promoter region. The cis-regulatory region of the rd22 promoter was identified by monitoring the expression of β-glucuronidas (GUS) activity in leaves of transgenic tobacco plants transformed with chimeric gene fusions constructed between 5′-deleted promoters of rd22 and the coding region of the GUS reporter gene. A 67-bp nucleotide fragment corresponding to positions − 207 to − 141 of the rd22 promoter conferred responsiveness to dehydration and ABA on a non-responsive promoter. The 67-bp fragment contains the sequences of the recognition sites for some transcription factors, such as MYC, MYB, and GT-1. The fact that accumulation of rd22 mRNA requires protein synthesis raises the possibility that the expression of rd22 might be regulated by one of these trans-acting protein factors whose de novo synthesis is induced by dehydration or ABA. Although the structure of the RD22 protein is very similar to that of a non-storage seed protein, USP, of Vicia faba, the expression of the GUS gene driven by the rd22 promoter in non-stressed transgenic Arabidopsis plants was found mainly in flowers and bolted stems rather than in seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293139
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  • 8
    Electronic Resource
    Electronic Resource
    The plant hormone abscisic acid mediates the drought-induced expression but not the seed-specific expression of rd22, a gene responsive to dehydration stress in Arabidopsis thaliana (1993)
    Springer
    Molecular genetics and genomics 238 (1993), S. 17-25 
    Details
    ISSN: 1617-4623
    Keywords: Arabidopsis thaliana ; Drought stress ; Abscisic acid ; Gene structure ; Seed protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nine cDNA clones, corresponding to genes that are responsive to dehydration (named RD), have been isolated from Arabidopsis thaliana. The sequence of a putative protein encoded by one of the RD cDNA clones, RD22, exhibits considerable homology to an unidentified seed protein (USP) of Vicia faba. Northern analysis showed that RD22 mRNA is induced by salt stress as well as by water deficit but not by cold or heat stress. RD22 mRNA appeared after the application of abscisic acid (ABA), an indication that transcription of RD22 mRNA is induced by endogenous ABA, the production of which is triggered by drought and salt stress. The induction of RD22 mRNA by ABA was inhibited by cycloheximide. Thus, it appears that protein synthesis is required for the induction of this mRNA by ABA. By contrast, protein synthesis was not required for the ABA-responsive induction RD29 mRNA, which corresponds to another dehydration-responsive gene of A. thaliana. These results suggest that there are at least two mechansisms for the induction of dehydration-responsive genes by ABA. RD22 mRNA was also expressed during the early and middle stages of seed development, showing a pattern of expression similar to that of USP. The seed-specific expression of RD22 seems not to be regulated by ABA. Structural analysis of the RD22 genomic clone revealed that the structural gene (designated rd22) contains three introns, and only a single copy of the gene is present in the A. thaliana genome, while the gene for USP from V. faba is actually a family of genes with 10 to 20 members. The site of initiation of transcription was determined by primer extension. Possible cis-acting elements involved in the expression of rd22 are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279525
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  • 9
    Electronic Resource
    Electronic Resource
    Two genes that encode Ca2+-dependent protein kinases are induced by drought and high-salt stresses in Arabidopsis thaliana (1994)
    Springer
    Molecular genetics and genomics 244 (1994), S. 331-340 
    Details
    ISSN: 1617-4623
    Keywords: Arabidopsis thaliana ; Protein kinase Water stress ; Ca2+-dependent activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two cDNA clones, AATCDPK1 and cATCDPK2, encoding Ca2+-dependent, calmodulin-independent protein kinases (CDPK) were cloned from Arabidopsis thaliana and their nucleotide sequences were determined. Northern blot analysis indicated that the mRNAs corresponding to the ATCDPK1 and ATCDPK2 genes are rapidly induced by drought and high-salt stress but not by low-temperature stress or heat stress. Treatment of Arabidopsis plants with exogenous abscisic acid (ABA) had no effect on the induction of ATCDPK1 or ATCDPK2. These findings suggest that a change in the osmotic potential of the environment can serve as a trigger for the induction of ATCDPK1 and ATCDPK2. Putative proteins encoded by ATCDPK1 and ATCDPK2 which contain open reading frames of 1479 and 1488 bp, respectively, are designated ATCDPK1 and ATCDPK2 and show 52% identity at the amino acid sequence level. ATCDPK1 and ATCDPK2 exhibit significant similarity to a soybean CDPK (51 % and 73%, respectively). Both proteins contain a catalytic domain that is typical of serine/threonine protein kinases and a regulatory domain that is homologous to the Ca2+-binding sites of calmodulin. Genomic Southern blot analysis suggests the existence of a few additional genes that are related to ATCDPK1 and ATCDPK2 in the Arabidopsis genome. The ATCDPK2 protein expressed in Escherichia coli was found to phosphorylate casein and myelin basic protein preferentially, relative to a histone substrate, and required Ca2+ for activation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286684
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  • 10
    Electronic Resource
    Electronic Resource
    Characterization of the expression of a desiccation-responsive rd29 gene of Arabidopsis thaliana and analysis of its promoter in transgenic plants (1993)
    Springer
    Molecular genetics and genomics 236 (1993), S. 331-340 
    Details
    ISSN: 1617-4623
    Keywords: Arabidopsis thaliana ; Desiccation-responsive gene ; Abscisic acid ; Promoter ; Transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We characterized the expression of genes that correspond to a cDNA clone, RD29, which is induced by desiccation, cold and high-salt conditions in Arabidopsis thaliana. Northern analysis of desiccation-induced expression revealed a two-step induction process. Early induction occurs within 20 min and secondary induction occurs 3 h after the start of desiccation. Exogenous abscisic acid (ABA) induces RD29 mRNA within 3 h. Two genes corresponding to RD29, rd29A and rd29B, are located in tandem in an 8 kb region of the Arabidopsis genome and encode hydrophilic proteins. Desiccation induces rd29A mRNA with two-step kinetics, while rd29B is induced only 3 h after the start of desiccation. The expression of both genes is stimulated about 3 h after application of ABA. It appears that rd29A has at least two cis-acting elements, one involved in the ABA-associated response to desiccation and the other induced by changes in osmotic potential. The β-glucuronidase (GUS) reporter gene driven by the rd29A promoter was induced at significant levels by desiccation, cold, high-salt conditions and ABA in both transgenic Arabidopsis and tobacco. Histochemical analysis of GUS activity revealed that the rd29A promoter functions in almost all the organs and tissues of vegetative plants during water deficiency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00277130
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