ISSN:
1573-5028
Keywords:
Arabidopsis
;
cloning
;
ribonucleoprotein
;
RNA-binding
;
RNP
;
sequence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have isolated a 1148 bp long cDNA clone encoding an RNA-binding protein in Arabidopsis. Several partial cDNA clones were isolated by screening an Arabidopsis λgt11 expression library for the binding of DNA. One of these clones was used as a probe to isolate a full-length clone. The 329 amino acid protein, termed RNP-T, contains in its carboxy terminus two adjacent RNP-80 motifs, a previously described 80 amino acid long conserved putative RNA-binding domain. Each RNP-80 motif includes both consensus short sequences, RNP1 and RNP2, which are separated by 33 amino acids. We have identified an acidic domain of 54 amino acids, which is located amino-terminal to the RNP-80 motifs. Seven tandem repeats of a hexamer are present within this domain. This acidic domain has a potential α-helix conformation. We propose that the acidic patch might play a role in protein-protein interaction.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00027154
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