ISSN:
1432-041X
Keywords:
Antisense RNA
;
BPV
;
Episomal expression vector
;
Actin gene
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary An expression vector was constructed containing the entire bovine papilloma virus (BPV-1) genome and part of the a-actin gene of Xenopus laevis cloned in the antisense orientation into the neomycin resistance gene under the control of the herpes simplex virus (HSV) thymidine kinase (TK) promoter. When this vector is microinjected into X. laevis embryos it replicates extrachromosomally, at least up to the tadpole stage, and a fusion RNA is synthesized after the mid blastula transition (MBT). The expression of the antisense gene results in a morphological abnormality of somites demonstrating that antisense RNA generated by an episomal replicating expression vector can inhibit the expression of a selected gene during early embryogenesis of X. laevis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00365121
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