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  • 1
    Publication Date: 1978-08-04
    Description: Neuronal cells, axons, and terminals containing immunoreactive enkephalin have been visualized in cultures of dissociated fetal spinal cord. These cultures may provide a valuable system in which to explore the effects of chronic drug treatment on the physiology of enkephalin-containing cells and their interactions with other cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Neale, J H -- Barker, J L -- Uhl, G R -- Snyder, S H -- New York, N.Y. -- Science. 1978 Aug 4;201(4354):467-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/351811" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Axons/metabolism ; Cells, Cultured ; Cytoplasm/metabolism ; Endorphins/*metabolism ; Enkephalins/*metabolism ; Fluorescent Antibody Technique ; Ganglia, Spinal/metabolism ; Mice ; Neurons/*metabolism ; Spinal Cord/cytology/embryology/*metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
    Publication Date: 1986-04-18
    Description: In situ hybridization of an oligonucleotide probe complementary to vasopressin messenger RNA (mRNA) in sections from normal or Brattleboro rat hypothalami revealed hybridization densities in each of three vasopressin-rich nuclei: the supraoptic, paraventricular, and suprachiasmatic. When entrained to a daily light-dark cycle, each rat strain displayed diurnal variation in hybridizable mRNA in the suprachiasmatic, but not in the supraoptic or paraventricular nuclei. The higher values for suprachiasmatic mRNA in the morning correlate well with previously elucidated morning increases in vasopressin immunoreactivity in the cerebrospinal fluid. These results support the utility of in situ hybridization techniques for elucidating physiological influences on regional peptidergic function, are consistent with a prominent role for vasopressinergic suprachiasmatic neurons in generating the cerebrospinal fluid vasopressin rhythm, and suggest that regulation of this mRNA rhythm is not dependent on release of intact peptide.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Uhl, G R -- Reppert, S M -- New York, N.Y. -- Science. 1986 Apr 18;232(4748):390-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3961487" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Autoradiography ; *Circadian Rhythm ; Nucleic Acid Hybridization ; Paraventricular Hypothalamic Nucleus/analysis/physiology ; RNA, Messenger/*analysis/isolation & purification ; Rats ; Rats, Brattleboro ; Rats, Inbred Strains ; Suprachiasmatic Nucleus/*analysis/physiology ; Supraoptic Nucleus/analysis/physiology ; Vasopressins/genetics/*physiology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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