Publication Date:
2007-11-22
Description:
Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yu, Junying -- Vodyanik, Maxim A -- Smuga-Otto, Kim -- Antosiewicz-Bourget, Jessica -- Frane, Jennifer L -- Tian, Shulan -- Nie, Jeff -- Jonsdottir, Gudrun A -- Ruotti, Victor -- Stewart, Ron -- Slukvin, Igor I -- Thomson, James A -- P20 GM069981/GM/NIGMS NIH HHS/ -- P51 RR000167/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Genome Center of Wisconsin, Madison, WI 53706-1580, USA. jyu@primate.wisc.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18029452" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Differentiation
;
*Cell Line
;
Cell Proliferation
;
Cell Shape
;
*Cellular Reprogramming
;
DNA-Binding Proteins/genetics/physiology
;
Embryonic Stem Cells/cytology
;
Fetus
;
Fibroblasts/*cytology
;
HMGB Proteins/genetics/physiology
;
Homeodomain Proteins/genetics/physiology
;
Humans
;
Infant, Newborn
;
Karyotyping
;
Mice
;
Mice, SCID
;
Octamer Transcription Factor-3/genetics/physiology
;
Oligonucleotide Array Sequence Analysis
;
Pluripotent Stem Cells/*cytology/physiology
;
RNA-Binding Proteins/genetics/physiology
;
SOXB1 Transcription Factors
;
Stem Cell Transplantation
;
Teratoma/pathology
;
Transcription Factors/genetics/physiology
;
Transduction, Genetic
;
Transgenes
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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