Publikationsdatum:
1997-02-21
Beschreibung:
Phototransduction in Drosophila occurs through the ubiquitous phosphoinositide-mediated signal transduction system. Major unresolved questions in this pathway are the identity and role of the internal calcium stores in light excitation and the mechanism underlying regulation of Ca2+ release from internal stores. Treatment of Drosophila photoreceptors with ryanodine and caffeine disrupted the current induced by light, whereas subsequent application of calcium-calmodulin (Ca-CaM) rescued the inactivated photoresponse. In calcium-deprived wild-type Drosophila and in calmodulin-deficient transgenic flies, the current induced by light was disrupted by a specific inhibitor of Ca-CaM. Furthermore, inhibition of Ca-CaM revealed light-induced release of calcium from intracellular stores. It appears that functional ryanodine-sensitive stores are essential for the photoresponse. Moreover, calcium release from these stores appears to be a component of Drosophila phototransduction, and Ca-CaM regulates this process.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Arnon, A -- Cook, B -- Montell, C -- Selinger, Z -- Minke, B -- EY03529/EY/NEI NIH HHS/ -- EY10852/EY/NEI NIH HHS/ -- New York, N.Y. -- Science. 1997 Feb 21;275(5303):1119-21.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology and the Kuhne Minerva Center for Studies of Visual Transduction, Hebrew University, Jerusalem 91120, Israel.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9027311" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Animals
;
Animals, Genetically Modified
;
Caffeine/pharmacology
;
Calcium/*metabolism
;
Calcium-Transporting ATPases/antagonists & inhibitors
;
Calmodulin/*metabolism
;
Drosophila/genetics/metabolism
;
Enzyme Inhibitors/pharmacology
;
In Vitro Techniques
;
*Light
;
Patch-Clamp Techniques
;
Photoreceptor Cells, Invertebrate/drug effects/*metabolism
;
Ryanodine/pharmacology
;
*Signal Transduction/drug effects
;
Thapsigargin/pharmacology
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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