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  • 1
    Electronic Resource
    Electronic Resource
    Isolation and characterization of microsatellites in trembling aspen (Populus tremuloides) (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 950-956 
    Details
    ISSN: 1432-2242
    Keywords: Key words Poplars (Populus) ; Simple sequencerepeats ; Microsatellite loci ; Polymorphism ; Clone identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have identified, isolated, and characterized microsatellite/simple sequence repeat (SSR) loci in trembling aspen (Populus tremuloides) by screening partial genomic libraries. We have also examined the compatibility and use of the P. tremuloides SSR primers to resolve microsatellites in other Populus species. Fourteen microsatellites were identified from 1600 clones screened. The TC/AG microsatellites were the most abundant. A total of 29 alleles were detected in 36 P. tremuloides individuals at the four SSR loci (two each of di- and tri-nucleotide repeats) characterized. The number of alleles at the SSR loci ranged from 5 to 11, with an average of 7.25 alleles per locus, and the observed heterozygosity ranged from 0.19 to 0.82, with a mean of 0.46 per locus. Although the highest polymorphism was observed for a dinucleotide SSR locus, the trinucleotide SSR loci showed substantial polymorphism. There were 34 unique multilocus genotypes among the 36 P. tremuloides individuals examined, and 89% of the individuals had unique multilocus genotypes. Two pairs of SSR primers were successful in PCR, amplifying genomic DNA and resolving microsatellites of comparable size from Populus deltoides, P. nigra, P.×canadensis, and P. maximowiczii. The microsatellite DNA markers developed could be used for clonal fingerprinting, certification of controlled crosses, genome mapping, marker-assisted early selection, genetic diversity assessments, and conservation and sustainable management of poplar genetic resources.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050825
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  • 2
    Electronic Resource
    Electronic Resource
    Sex allocation and mating systems in pigeonpea,Cajanus cajan (Fabaceae) (1989)
    Springer
    Plant systematics and evolution 168 (1989), S. 59-69 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Fabaceae ; pigeonpea ; Cajanus cajan ; Sex allocation ; mating systems ; fruit and seed set
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sex allocation theory predicts that: (1) resources allocated to androecium should decrease with an increase in selfing, (2) a decrease in androecium biomass should be accompanied by an increase in the biomass of pistils, and (3) a decrease in androecium biomass should be coupled with a decrease in flower size, specifically corolla biomass. Another predicted change in reproductive traits associated with variation in selfing concerns seed to ovule ratios, but does not directly stem from sex allocation theory. It has been postulated that seed to ovule ratios should be positively correlated with the amount of selfing. These predictions were tested for six accessions of pigeonpea,Cajanus cajan L., that differed in selfing rates. The results were remarkably in accordance with the predictions. We conclude that sex allocation theory provides a powerful tool to understand the evolution of many reproductive traits in plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00936107
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  • 3
    Electronic Resource
    Electronic Resource
    Patterns of fruit and seed production inBauhinia ungulata (Leguminosae) (1985)
    Springer
    Plant systematics and evolution 151 (1985), S. 55-65 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Leguminosae ; Caesalpinioideae ; Bauhinia ungulata. — Fruit production ; seed production ; position effects ; herbivory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Patterns of seed and fruit production ofBauhinia ungulata, a small tree legume indigenous in tropical America, were studied in Costa Rica. Only about 8% of flowers produced fruits. The average pod had 19 ovules and about two thirds of these began seed development, with mature pods containing an average of 9.7 mature undamaged seeds. About half of the mature pods were damaged by herbivores and within these, 27% of ovules or seeds had been eaten. Among trees there was no significant variation in pod production, but the number of ovules per pod and seed production per pod varied significantly. Within infructescences most pods were retained at middle positions. Within pods, the probability of an ovule developing into a seed increased toward the distal end. The pattern of seed and fruit production in this species agrees well in general with that reported for other neotropical legumes. The abortion of seeds and fruits can be regarded as a way of controlling maternal investment, and as a response to herbivory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02418019
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