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  • 1
    Electronic Resource
    Electronic Resource
    Nuclear magnetic resonance microscopy ofAncistrocladus heyneanus (1997)
    Springer
    Protoplasma 198 (1997), S. 210-217 
    Details
    ISSN: 1615-6102
    Keywords: Nuclear magnetic resonance micro-imaging ; Chemical-shift imaging ; Fluorescence microscopy ; Ancistrocladus heyneanus ; Ancistrocladaceae ; Naphthylisoquinoline alkaloids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The tropical lianaAncistrocladus heyneanus, which is known for its biologically active naphthylisoquinoline alkaloids, has been studied by nuclear magnetic resonance (NMR) microscopy for the first time. The spatial resolution of the cross-sectional NMR images was of the order of 20 μm. Quantitative NMR relaxation time images of the root and the shoot show great contrast between different tissue regions. In addition, we observed the regional distribution of chemical compounds inAncistrocladus heyneanus by chemical-shift NMR microscopy. The NMR imaging results were compared with light and fluorescence microscopic images and reveal the excellent tissue characterization using NMR technology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287570
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  • 2
    Electronic Resource
    Electronic Resource
    FT-Raman investigation of alkaloids in the liana Ancistrocladus heyneanus (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biospectroscopy 4 (1998), S. 113-120 
    Details
    ISSN: 1075-4261
    Keywords: FT-Raman spectroscopy ; micro-Raman technique ; Ancistrocladus heyneanus ; naphthylisoquinoline alkaloids ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The applicability of the micro-FT-Raman technique for studying alkaloids in vitro and for observing alkaloids in plant cells is demonstrated. This technique is examined using fresh plant material of Ancistrocladus heyneanus, a tropical liana known to produce pharmacologically interesting naphthylisoquinoline alkaloids as secondary metabolites. It will be shown that it is possible to localize and identify some of these alkaloids in different parts of the plant by means of Raman microspectroscopic studies. Data on the in situ structure and the spatial distribution can be obtained, which could provide information about the biosynthesis of the alkaloids in the plant. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: 113-120, 1998
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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