ISSN:
1052-9306
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A sensitive gas chromatographic/mass spectrometric assay has been developed to quantify 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 1-methyl-4-phenylpyridinium ion (MPP+) in brain tissue. MPTP was derivatized with isobutyl chloroformate and then analyzed using gas chromatography/mass spectrometry. MPP+, a known metabolite of MPTP, was reduced with NaB2H4 to 2H2-MPTP before derivatization. Deuterated isotopomers of MPTP and MPP+ were used as internal standards. The intra-assay coeffients of variation in the determination of 57.3 ng MPTP and 371.8 ng MPP+ in 100 mg of mouse brain tissue were 3.0% and 5.6% (n = 7), respectively. The detection limit corresponds to about 2 ng per sample of authentic MPTP.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200130207
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