ISSN:
0749-1581
Keywords:
Scleroglucan
;
13C NMR
;
Magic angle spinning
;
Relaxation times
;
Solid state
;
Hydration
;
Gel
;
Solution
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Scleroglucan is a branched poiysaccharide, soluble in water. Solid-state and solution 13C NMR were used to follow the fixation of water molecules on anhydrous scleroglucan and the evolution of the scleroglucan - water complex from the solid state to the gel and solution state. Determination of both chemical shift values and relaxation times (TCHD, T1pH and T1C) for anhydrous and hydrated scleroglucans permit the observation of a transition between the two forms. The fixation of water leads to an increase in the solid-state order, in the form of a triple-helix association, and at the same time an increase in the interchain distance and in the molecular motion. The gel state obtained for a water-scleroglucan composition of 90:10 is characterized by an important macromolecular rigidity which implies the use of the solid-state NMR techniques. Solution 13C NMR can be utilized to characterize the primary structure and the triple-helix random-coil conformational transition.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1260311003
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