ISSN:
1052-9306
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A selected ion monitoring method for the determination of 6-oxo-PGF1α, the stable end-product of prostacyclin, in biological fluids has been developed. In this method, biosynthetically prepared [2H6]-6-oxo-PGF1α is used as internal standard. The method involves extraction, thin-layer chromatography purification and derivatization into the methyl ester, methoxime, trimethylsilyl ether derivatives by carrying out the methoximation first. Quantitative gas chromatographic mass spectrometric analysis is performed in the electron impact mode by monitoring the [M - (TMSOH + CH3O.)]+. fragment ions. The use of this method in the measurement of 6-oxo-PGF1α in serous fluids and in incubation media of serous tissues is described.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200071119
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