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  • Analytical Chemistry and Spectroscopy  (7)
  • 1
    ISSN: 0886-9383
    Keywords: NIR ; Fast Fourier transform ; Principal component analysis ; Discrimination ; Baking quality ; Wheat ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Digitalized continuous near infra-red reflectance (NIR) spectra are composed of a great number of data which must be reduced for microcomputer mathematical treatment. The sequence ‘fast Fourier transform preceding principal component analysis’ was tested to perform data size reduction without a large loss of information. The method was applied on a collection of wheat spectra composed of 351 data. Ten resulting data, which described 99.5% of the total variance, were kept. The relevance of the method was estimated by the ability of the resulting data (i) to regenerate the original signal, and (ii) to discriminate the baking quality of the wheat by stepwise multiple discriminant analysis. The average difference between initial and regenerated spectra was -2.4 × 10-3 log (1/R) units and the standard deviation was 1.16 × 10-3 log (1/R) units. The discrimination treatments gave 89.9% of well classified samples for the calibration test and 90.5% for the prediction test. The application of these mathematical treatments to other continuous signals is discussed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Chemometrics 6 (1992), S. 163-175 
    ISSN: 0886-9383
    Keywords: Multiple-correspondence analysis ; Starch granule description ; Image analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Raw starch is composed botanically of characteristic granules of various sizes and shapes, so that each kind of starch may be characterized by the population of its granules. In the present study ten commercial starch species were studied: wheat, rice, manioc, potato, arrowroot, amylomaize, normal maize, waxy maize and two different banana species. Six variables measuring the size and shape of granules were obtained by image analysis. The objective was to find a method to describe and compare the granule populations of the ten species. For such a study, multiple-correspondence analysis (MCA) was applied. MCA makes it possible to draw similarity maps of categories and objects. For each starch species the frequency distributions (histograms) of the six variables were assessed and each granule was characterized by its species and the classes of histograms to which it belonged. MCA was applied to the granule table and a description of the histogram classes and the granules was obtained. From the variables description a general typology of the granules was deduced. The similarity maps showed considerable scatter of the granules for all species except rice. A particular species could therefore not be identified by a single granule, but the granule distribution seemed to be characteristic. MCA was an appropriate method to analyse these data because it points out non-linear relationships between quantitative and qualitative variables.
    Additional Material: 7 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Chemometrics 9 (1995), S. 125-135 
    ISSN: 0886-9383
    Keywords: Procrustean analysis ; FT-IR spectroscopy ; NIR spectroscopy ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Procrustean methods allow the fitting of a given matrix to another given matrix observed on the same objects. In the traditional approach orthogonal constraints are imposed upon the transformation matrix, whereas in the alternative approach Procrustean analysis may be performed without such constraints. The two methods (with and without constraints) were compared on data dealing with mid- and near-infrared spectra of oil. The aim was to reconstruct the mid-infrared spectral information using data from the near-infrared spectra. Unconstrained Procrustean analysis proved to be the more efficient for both the calibration and verification sets. Furthermore, the analysis of the transformation matrix between the two infrared ranges made it possible to indicate wavelengths and wave numbers corresponding to the same chemical groups.
    Additional Material: 7 Ill.
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  • 4
    ISSN: 0886-9383
    Keywords: NIR reflectance ; Multivariate analysis ; Principal component analysis ; Stepwise multiple linear regression ; Silage quality ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Calibrations to predict crude protein (CP) and in vitro dry matter digestibility (IVDMD) in dried grass silage from reflectance data collected at 19 wavelengths on an InfraAlyzer 400R have been developed using stepwise multiple linear (SML) and principal component (PC) regression techniques. A direct comparison of the efficacy of each multivariate technique in this applications has been possible by using identical calibration development and evaluation sample sets. The effect of two data transformation steps prior to PC regression was also investigated. PC regression of raw reflectance data yielded no significant improvement in the standard errors of prediction (SEP) for CP and IVDMD over those obtained by SMLR, viz. 0·61 vs 0·63 and 2·9 vs 3·0 respectively. Computation time for development and evaluation of the PC regression equation was less than for selection of the best SMLR equation, and PCR equations may be more robust. Data transformation to reduce granularity effects prior to PCR did not produce any improvement in predictive accuracy for either IVDMD or CP.
    Additional Material: 5 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 5 (1991), S. 299-302 
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The molecular weights of the recombinant proteins interleukin 2 and interferon gamma were determined by electrospray ionization (ESI) mass spectroscopy on the whole molecules. For interleukin 2 an average experimental mass of 15 549.4 u, was observed in excellent agreement with the theoretical average mass of 15 547.2 u. The mass observed for interferon gamma (16 908.4 u) agreed with the theoretical molecular weight of the monomeric species (16 907.3 u). By ESI mass spectrometry it was possible to determine the presence of proteins lacking one or two amino acids.
    Additional Material: 4 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 19 (1996), S. 535-542 
    ISSN: 0935-6304
    Keywords: On-line LC-GC coupling ; Fully concurrent eluent evaporation ; Sample pretreatment ; Residue analysis ; Complex biological matrices ; Pyrethroid insecticide ; Acrinathrin ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The following paper presents a fast, selective, sensitive, and automated procedure for the residue analysis of the pyrethroid insecticide acrinathrin in various fruit samples by on-line combination of Liquid Chromatography (LC) with capillary Gas Chromatography (GC) and an Electron-Capture Detector (ECD). The loop-type interface and Fully Concurrent Solvent Evaporation (FCSE) were chosen due to their ease of use and low volatility of acrinathrin. Commercially available LC diol columns with small internal diameters (2.1 mm) provided the selectivity required for the separation of the solute from matrix components and permitted the transfer of fraction volumes up to 500 μl into the retention gap, located inside the gas chromatograph. Optimization of the operating conditions with standard solutions showed the influence of the GC initial transfer temperature on the response of acrinathrin and the quantitative nature of the transfer was established by liquid scintillation counting. The linearity and precision were studied with the aid of calibration plots, whereas quantitative recoveries of the analyte (at least 95 %) were determined in spiked fruits. Compared to the corresponding off-line technique, the on-line approach allows us to reach lower detection limits (ppb level when 20 μl LC injections are performed) with excellent repeatability between 0.6 and 6.6 %. Moreover, the integrated analytical set-up is robust, less time-consuming and subsequently brings a marked improvement in the sample preparation step of complex biological matrices. Finally, the versatility of the system renders it highly suitable for the determination of other similar pyrethroids in order to develop a multiresidue method.
    Additional Material: 6 Ill.
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  • 7
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Ill.
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