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  • Analytical Chemistry and Spectroscopy  (2)
  • Isolated organelles  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Faithful and highly efficient RNA synthesis in isolated mitochondria from rat liver (1988)
    Springer
    Current genetics 14 (1988), S. 477-482 
    Details
    ISSN: 1432-0983
    Keywords: Non-growing cells ; Isolated organelles ; RNA synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Isolated rat liver mitochondria in the presence of an appropriate incubation buffer are able to support DNA transcription and RNA processing in a way qualitatively and quantitatively similar to that used by intact cells. This system is also able to synthesize an RNA species of 155–175 nucleotides which probably corresponds to the 7S RNA, a type of RNA found so far only in growing cells. The role of this RNA in the mitochondrial replication and transcription processes, in relation to the cell metabolism, is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00521272
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of biological samples by two-dimensional infrared spectroscopy: Simulation of frequency, bandwidth, and intensity changes (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biospectroscopy 2 (1996), S. 341-351 
    Details
    ISSN: 1075-4261
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Two-dimensional infrared (2D IR) spectroscopy has been shown to be a powerful tool for the analysis of spectra with highly overlapped bands, as often found in IR spectra of biological samples. To date, most 2D IR analyses have focused primarily on intensity changes of the bands under investigation. However, information concerning 2D IR characteristics of bands that change in position or width is sparse. We have thus simulated the effects of frequency and bandwidth changes on 2D IR spectra. In the synchronous plot of a band undergoing a frequency shift, two autopeaks and two crosspeaks are found at the initial and final positions, while the asynchronous plot exhibits two weaker crosspeaks for these positions and a stronger, somewhat elongated feature close to the diagonal. The latter feature is characteristic of a shifting band. Thus, to distinguish a frequency shift in a single band from intensity changes of two overlapped bands it is important to examine the asynchronous plot, since the synchronous plots exhibit comparable characteristics in both cases. A bandwidth change results in a series of crosspeaks. However, when bandwidth changes are coupled with either frequency shifts and/or intensity changes, the effect of the bandwidth change is reduced. Finally, it is shown that the resolution enhancement generally found for the asynchronous plot is accompanied by an error in the positions of the original spectral features as determined from 2D IR peaks. The magnitude of the error increases as the original spectral features approach each other in frequency. © 1996 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Two-dimensional infrared correlation spectroscopy of synthetic and biological apatites (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biospectroscopy 2 (1996), S. 353-364 
    Details
    ISSN: 1075-4261
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Two-dimensional (2D) infrared (IR) correlation spectroscopy was used to monitor the ν1, ν3 phosphate contour (900-1200 cm-1) of maturing poorly crystalline hydroxyapatite in synthetic (synthesized at constant and variable pH) and biological (calcified turkey leg tendon) systems. The 2D IR plots of the mineral prepared at variable pH exhibit peaks at 961, 999, 1018, 1036, 1095, 1126, and 1150 cm-1. The peaks at 961, 999, and 1095 cm-1 represent vibrations of PO3-4 in an apatitic/stoichiometric environment of poorly crystalline HA, while those at 1018, 1036, and 1126 cm-1 arise from PO3-4 in a nonstoichiometric/acid phosphate environment of poorly crystalline HA. The 2D IR analysis suggests that the intensities of peaks associated with PO3-4 in a nonstoichiometric/acid phosphate environment decrease as the reaction progresses. The 2D IR plots of the mineral formed at constant pH showed only bands characteristic of PO3-4 in a stoichiometric/acid phosphate environment. Analysis of the 2D IR plots of the mineral from calcified turkey leg tendon reveals peaks at 1019, 1039, 1075, 1126, and 1147 cm-1. The peaks at 1019, 1039, and 1126 cm-1 are characteristic of PO3-4 in a nonstoichiometric/acid phosphate environment of poorly crystalline HA, while the band at 1075 cm-1 is characteristic of PO3-4 in an apatitic/stoichiometric environment of poorly crystalline HA. Thus, the in vitro experiment in which the mineral is formed at variable pH is a better model of the mineral phase in calcified turkey leg tendon. In addition, the asynchronous plots from both the synthetic and biological minerals revealed those peaks which were noncorrelated. Also, this method of data analysis provided enhanced resolution of the highly overlapped ν1, ν3 phosphate contour commonly seen in Fourier transform-IR spectra of calcified tissue. © 1996 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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