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  • Analytical Chemistry and Spectroscopy  (3)
  • Echinoderms  (2)
  • 1985-1989  (5)
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  • 1985-1989  (5)
Year
  • 1
    ISSN: 1432-1432
    Keywords: Histone genes ; Echinoderms ; Maternal mRNA ; Development ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The organization and maternal expression of histone genes differ greatly between the two echinoderm classes represented by the sea stars and sea urchins. We have isolated and characterized a 5.3-kb genomic DNA fragment encoding core histone genes in the sea starPisaster ochraceus. The majority of histone genes are organized as tandem repeats of the 5.3-kb fragment, which is present in as many as 700 copies per haploid genome. The identity, precise location, and transcriptional polarity of individual core histone genes within the repeat were determined by DNA sequence analyses. The gene order in the sea star (H2B, H2A, H4, H3) is different from that in sea urchins (H2B, H3, H2A, H4). What is remarkable is the low level of maternal histone mRNA inP. ochraceus eggs relative to that in sea urchins. This observation supports a recent suggestion that major changes in mode of gene expression, rather than in gene organization or copy number, can be correlated with major events in echinoderm evolution.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1432
    Keywords: Histone genes ; Sea stars ; Echinoderms ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The arrangement of core histone genes and their transcriptional polarity has been determined for three species of sea stars (Pisaster ochraceus, P. brevispinus, andDermasterias imbricata) representing two orders which diverged over 500 million years ago. Each species has approximately 500 core histone cluster repeats per haploid genome. The close phylogenetic relationship between thePisaster species is evident from the correspondence of restriction sites in the repeat element, identical arrangement of core histones, and high degree of sequence homology in both the coding and spacer regions of the H3 gene. TheDermasterias repeat has the same gene order and transcriptional polarity of core histones, but its restriction map is significantly different. Moreover, theDermasterias H3 gene has the same amino acid sequence, but in comparison toPisaster nucleotide sequences, shows a high level of silent substitutions. Analyses of the nucleotide sequence of the 5′ and 3′ regions surrounding the H3 gene from each species demonstrate the presence of appropriately spaced consensus and processing signal segments. The 3′ spacer segment of theDermasterias H4 gene contains an unusual, threefold tandemly repeated, 21-nucleotide, AT-rich sequence. No similar sequence is seen in theP. brevispinus H4 3′ region, but these two species show a striking regularity of distribution of five different homologous oligomers in the 3′ spacer.
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  • 3
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Positive ion fast atom bombardment mass spectra of maltooligosaccharides reductively aminated with 2-aminopyridine (Gn-AP) contain abundant [M + H]+ ions. Determination of G2-AP and G3-AP produced from G5-AP by the action of α-amylases, based on the abundance of their [M + H]+ ions relative to that of cellobiose reductively aminated with 2-amino-6-methylpyridine as the internal standard, allowed rapid and reproducible assay of these enzymes. It was advantageous for clinical investigation that the proportion of pancreatic and salivary α-amylase activities could be determined.
    Additional Material: 4 Ill.
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  • 4
    ISSN: 0749-1581
    Keywords: Conformation ; D:C-friedoolean-8-enes ; Molecular mechanics calculation ; 1H and 13C NMR ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Comparison of the 1H and 13C chemical shift data of bryonolic acid (D : C-friedoolean-8-en-3β-ol-29-oic acid) and its derivatives (13 species) revealed that the conformational features of these compounds in CDCl3 solution depend on whether the functional groups at C-29 are trigonal, such as COOCH3, or tetrahedral, such as CH2OH. In order to study the conformational features of these two groups of compounds, vicinal coupling constants between the protons on the E rings of the methyl ester of D : C-friedoolean-8-en-29-oic acid, D : C-friedoolean-8-en-29-oic and its acetate were measured. Comparison of the results with the steric geometries to be expected from the molecular mechanics calculation for the E-ring moieties of these derivatives in the S form (D-E rings: boat-boat form) and the F form (D-E rings: chair-chair form) led to the conclusion that the compounds having a trigonal functional group at C-29 mainly adopt the S form, and those having a tetrahedral functional group at C-29 are present as mixtures of the S and F form in equilibrium in the solutions.
    Additional Material: 9 Ill.
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  • 5
    ISSN: 0749-1581
    Keywords: 13C-enrichment ; 13C NMR ; spectral assignment ; Cycloartanol ; Cycloartanol ; Skeleton formation mechanism ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Previously proposed 13C NMR spectral assignments of cycloartenol (9,19-cyclolanost-24-en-3β-ol) (1) and cycloartanol (9,19-cyclolanostan-3β-3-ol) (2) were re-examined by means of 13C-enrichment experiments and LIS measurement of 13C chemical shifts. Revised signal assignments are proposed for C-7, C-11, C-16, C-18, C-21 and C-28 of 1 and C-7, C-11, C-12, C-15 and C-16 of 2. Experimental proof for the skeleton formation mechanism of 1 based on 13C NMR spectral findings is presented.
    Additional Material: 2 Ill.
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