ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Analgesic peptides
;
Opioids
;
Glycopeptides
;
Glycosylenkephalins
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary N 1.5-Glycosyl enkephalins are potent analgesics. Two enkephalin-related glycosyl peptides: [DMet2, Pro5] enkephalin (N 1.5 β-glucopyranosyl] amide and [DMet2, Pro5] enkephalin [N 1.5 β-galactosyl] amide have been prepared following the liquid phase procedure. The chromatographic behavior of both the final glycosyl enkephalins and their synthetic intermediates on reversed-phase columns has been tested. Semipreparative, reversed-phase, HPLC conditions have been developed in order to obtain a simple and rapid procedure for the purification of the two synthetic pentapeptides. Thus, using isocratic elution, injections of 10mg of crude material after gel filtration gave homogeneous (99.9%) glycosyl enkephalins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02311424
Permalink