ISSN:
1432-072X
Keywords:
Key words Thermotoga neapolitana
;
Thermophile
;
Anaerobe
;
Auxotrophs
;
Antimetabolites
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Antimetabolite-resistant and auxotrophic mutants of the hyperthermophilic bacterium Thermotoga neapolitana were isolated to provide strains with genetic backgrounds amenable to genetic analyses. Norleucine, azaleucine, 4-nitropyridine-N-oxide, and 3-amino-1, 2, 4-triazole did not affect growth, while 5-fluorouracil (5 μg/ml), 5-methyltryptophan (250 μg/ml), 6-azauracil (100 μg/ml), and 4-fluorophenylalanine (30 μg/ml) inhibited growth at the indicated minimum inhibitory concentrations. The effect of 5-fluorouracil was analyzed and found to be bacteriostatic. These inhibitors were used to select spontaneously arising resistant mutants. In addition, auxotrophic mutants requiring leucine, tryptophan, adenine, and histidine were isolated following mutagenesis with ethyl methanesulfonate. Six other auxotrophs with undefined growth requirements were also isolated. These strains will be useful for the development of genetic methods for T. neapolitana.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002030050150
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